Sutjarit Nareerat, Ruknarong Laongthip, Tanprasertkul Chamnan, Bhukhai Kanit, Soe Hay Man Saung Hnin, Kheolamai Pakpoom, Manochantr Sirikul, Tantrawatpan Chairat, Tantikanlayaporn Duangrat
Nutrition Unit, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand.
Center of Excellence in Stem Research and Innovation, Thammasat University, Pathumthani, 12120, Thailand.
Sci Rep. 2025 Aug 12;15(1):29477. doi: 10.1038/s41598-025-15647-9.
Umbilical cord blood (UCB) units are an alternative source of human hematopoietic stem cells (HSCs) for allogeneic stem cell transplants. A large quantity of HSCs is needed but the low number of accessible cells from UCB has been a significant limitation. Improving the ex vivo growth of HSCs while preserving their functioning is required. Here, we report that andrographolide (AP) enhanced the expansion of human UCB-derived HSCs (HSPCs) and pro-moted primitive HSCs (CD34CD38CD90). AP also improved HSC functionality, evidenced by increased growth of colony-forming units and multilineage differentiation. AP upregulated genes involved in the Wnt/β-catenin and Notch signaling pathways. AP also modulated signaling pathways involved in HSC self-renewal, proliferation, survival, and differentiation, demonstrated by Nanostring analysis. The results of this study suggest that andrographolide enhances ex vivo UCB-HSC expansion while maintaining functionality and has potential for treatment of hematological diseases.
脐带血（UCB）单位是用于异基因干细胞移植的人类造血干细胞（HSCs）的替代来源。异体干细胞移植需要大量的造血干细胞，但从脐带血中获取的细胞数量较少一直是一个重大限制。需要在保持造血干细胞功能的同时提高其体外生长能力。在此，我们报告穿心莲内酯（AP）可增强人脐带血来源的造血干细胞（HSPCs）的扩增，并促进原始造血干细胞（CD34+CD38−CD90+）的生长。穿心莲内酯还改善了造血干细胞的功能，集落形成单位的生长增加和多系分化证明了这一点。穿心莲内酯上调了参与Wnt/β-连环蛋白和Notch信号通路的基因。通过纳米串分析表明，穿心莲内酯还调节了参与造血干细胞自我更新、增殖、存活和分化的信号通路。本研究结果表明，穿心莲内酯可增强体外脐带血造血干细胞的扩增，同时保持其功能，具有治疗血液疾病的潜力。
