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穿心莲内酯促进源自人脐带血的CD34+造血干细胞的体外扩增。

Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood.

作者信息

Sutjarit Nareerat, Ruknarong Laongthip, Tanprasertkul Chamnan, Bhukhai Kanit, Soe Hay Man Saung Hnin, Kheolamai Pakpoom, Manochantr Sirikul, Tantrawatpan Chairat, Tantikanlayaporn Duangrat

机构信息

Nutrition Unit, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand.

Center of Excellence in Stem Research and Innovation, Thammasat University, Pathumthani, 12120, Thailand.

出版信息

Sci Rep. 2025 Aug 12;15(1):29477. doi: 10.1038/s41598-025-15647-9.

DOI:10.1038/s41598-025-15647-9
PMID:40796650
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12343817/
Abstract

Umbilical cord blood (UCB) units are an alternative source of human hematopoietic stem cells (HSCs) for allogeneic stem cell transplants. A large quantity of HSCs is needed but the low number of accessible cells from UCB has been a significant limitation. Improving the ex vivo growth of HSCs while preserving their functioning is required. Here, we report that andrographolide (AP) enhanced the expansion of human UCB-derived HSCs (HSPCs) and pro-moted primitive HSCs (CD34CD38CD90). AP also improved HSC functionality, evidenced by increased growth of colony-forming units and multilineage differentiation. AP upregulated genes involved in the Wnt/β-catenin and Notch signaling pathways. AP also modulated signaling pathways involved in HSC self-renewal, proliferation, survival, and differentiation, demonstrated by Nanostring analysis. The results of this study suggest that andrographolide enhances ex vivo UCB-HSC expansion while maintaining functionality and has potential for treatment of hematological diseases.

摘要

脐带血（UCB）单位是用于异基因干细胞移植的人类造血干细胞（HSCs）的替代来源。异体干细胞移植需要大量的造血干细胞，但从脐带血中获取的细胞数量较少一直是一个重大限制。需要在保持造血干细胞功能的同时提高其体外生长能力。在此，我们报告穿心莲内酯（AP）可增强人脐带血来源的造血干细胞（HSPCs）的扩增，并促进原始造血干细胞（CD34+CD38−CD90+）的生长。穿心莲内酯还改善了造血干细胞的功能，集落形成单位的生长增加和多系分化证明了这一点。穿心莲内酯上调了参与Wnt/β-连环蛋白和Notch信号通路的基因。通过纳米串分析表明，穿心莲内酯还调节了参与造血干细胞自我更新、增殖、存活和分化的信号通路。本研究结果表明，穿心莲内酯可增强体外脐带血造血干细胞的扩增，同时保持其功能，具有治疗血液疾病的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/6bd0fdac80e6/41598_2025_15647_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/6e0740fcdbe5/41598_2025_15647_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/beb5ee3c7fd1/41598_2025_15647_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/fdc074c4e458/41598_2025_15647_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/cf1f4a9b0a20/41598_2025_15647_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/faaf3d241340/41598_2025_15647_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/49ead3a850a9/41598_2025_15647_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/6bd0fdac80e6/41598_2025_15647_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/6e0740fcdbe5/41598_2025_15647_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/beb5ee3c7fd1/41598_2025_15647_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/fdc074c4e458/41598_2025_15647_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/cf1f4a9b0a20/41598_2025_15647_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/faaf3d241340/41598_2025_15647_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/49ead3a850a9/41598_2025_15647_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7920/12343817/6bd0fdac80e6/41598_2025_15647_Fig7_HTML.jpg

相似文献

[1]
Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood.

Sci Rep. 2025-8-12

[2]
Endogenous Hydrogen Sulphide Promotes the Ex Vivo Expansion of Haematopoietic Stem Cells by Regulating the Activation of the JAK2/STAT3 Pathway.

Immunology. 2025-8

[3]
Mesenchymal stem/stromal cells from human pluripotent stem cell-derived brain organoid enhance the ex vivo expansion and maintenance of hematopoietic stem/progenitor cells.

Stem Cell Res Ther. 2024-3-5

[4]
Umbilical cord blood derived cell expansion: a potential neuroprotective therapy.

Stem Cell Res Ther. 2024-7-29

[5]
Robust Expansion of Hematopoietic Stem Cells Ex Vivo Using Small Molecule Cocktails.

Methods Mol Biol. 2025

[6]
Differential ability of MSCs isolated from placenta and cord as feeders for supporting ex vivo expansion of umbilical cord blood derived CD34(+) cells.

Stem Cell Res Ther. 2015-10-19

[7]
Synergistic effect and molecular mechanism of PVA and UM171 in ex vivo expansion of primitive hematopoietic stem cells.

J Cell Biochem. 2024-1

[8]
Ex vivo expansion and functional activity preservation of adult hematopoietic stem cells by a diarylheptanoid from Curcuma comosa.

Biomed Pharmacother. 2021-11

[9]
Efficient Expansion of SALL4-Transduced Umbilical Cord Blood Derived CD133+Hematopoietic Stem Cells.

Acta Med Iran. 2017-5

[10]
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid.

J Vis Exp. 2019-4-11

本文引用的文献

[1]
The Role of the Notch Signaling Pathway in the Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells.

Front Biosci (Landmark Ed). 2024-2-21

[2]
Ex vivo expansion of human hematopoietic stem cells and clinical applications.

Cancer Sci. 2024-3

[3]
Ex vivo hematopoietic stem cell expansion technologies: recent progress, applications, and open questions.

Exp Hematol. 2024-2

[4]
Emerging role of Hippo pathway in the regulation of hematopoiesis.

BMB Rep. 2023-8

[5]
Wnt regulation of hematopoietic stem cell development and disease.

Curr Top Dev Biol. 2023

[6]
Crosstalk between the Hippo Pathway and the Wnt Pathway in Huntington's Disease and Other Neurodegenerative Disorders.

Cells. 2022-11-16

[7]
The Hippo signalling pathway and its implications in human health and diseases.

Signal Transduct Target Ther. 2022-11-8

[8]
Hematopoietic stem cell transplantation and cellular therapies for autoimmune diseases: overview and future considerations from the Autoimmune Diseases Working Party (ADWP) of the European Society for Blood and Marrow Transplantation (EBMT).

Bone Marrow Transplant. 2022-7

[9]
Ex vivo expansion and functional activity preservation of adult hematopoietic stem cells by a diarylheptanoid from Curcuma comosa.

Biomed Pharmacother. 2021-11

[10]
Ex vivo expansion of hematopoietic stem cells: Finally transitioning from the lab to the clinic.

Blood Rev. 2021-11

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