Hosoya Makoto, Suda Satoshi, Ueno Masafumi, Shimanuki Marie N, Nishiyama Takanori, Oishi Naoki, Ozawa Hiroyuki
Department of Otorhinolaryngology, Head and Neck Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Sci Rep. 2025 Aug 12;15(1):29527. doi: 10.1038/s41598-025-15685-3.
Membrane channels play an important role in auditory processes. Connexins and pannexins are membrane channels that exist in the cochlea. Connexin26 and connexin30 have been previously shown to be differentially expressed in the developing cochlea of primates in a species-specific manner. However, whether other connexins and pannexins are also expressed in the developing primate cochlea has not been investigated. In this study, we sought to examine the expression patterns of connexin43, connexin31, and pannexin1 during cochlear development in a primate animal model, the common marmoset, to determine the differences observed between the marmoset and existing rodent models. Our observations revealed both interspecies differences in the expression patterns of these genes and similarities between rodents and primates. These results indicate that the spatiotemporal patterns of connexin and pannexin expression are complex and differ between rodents and primates. They also highlight the importance of carefully observing differences in developmental changes in connexins and pannexins between humans and rodents.
膜通道在听觉过程中发挥着重要作用。连接蛋白和泛连接蛋白是存在于耳蜗中的膜通道。先前已表明，连接蛋白26和连接蛋白30在灵长类动物发育中的耳蜗中以物种特异性方式差异表达。然而，尚未研究其他连接蛋白和泛连接蛋白是否也在发育中的灵长类动物耳蜗中表达。在本研究中，我们试图在灵长类动物模型普通狨猴中研究连接蛋白43、连接蛋白31和泛连接蛋白1在耳蜗发育过程中的表达模式，以确定狨猴与现有的啮齿动物模型之间观察到的差异。我们的观察结果揭示了这些基因表达模式的种间差异以及啮齿动物和灵长类动物之间的相似性。这些结果表明，连接蛋白和泛连接蛋白表达的时空模式很复杂，并且在啮齿动物和灵长类动物之间存在差异。它们还强调了仔细观察人类和啮齿动物之间连接蛋白和泛连接蛋白发育变化差异的重要性。
