Zhao Yanfei, Yan Na, Yang Jintao, Jiang Xinhua
Department of Laboratory Medicine, Quzhou Maternal and Child Health Care Hospital, Quzhou, Zhejiang Province, China.
Key Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Dian Diagnostics Group Co., Ltd., Hangzhou, Zhejiang Province, China.
Medicine (Baltimore). 2025 Aug 8;104(32):e43780. doi: 10.1097/MD.0000000000043780.
This study investigates the correlation between circulating hsa-miR-21-5p, hsa-miR-133a-3p, hsa-miR-182-5p, and bone density levels in human plasma. A total of 120 subjects with normal bone mass, osteopenia, and osteoporosis were recruited. RT-PCR was used to detect the relative expression levels of hsa-miR-21-5p, hsa-miR-133a-3p, hsa-miR-182-5p in the plasma of the patients, and the correlation analysis was performed combined with physical examination data. Analyze the potential effects of hsa-miR-21-5p and hsa-miR-133a-3p on bone metabolism using bioinformatics tools. Compared with the normal group, the expression of plasma hsa-miR-21-5p was significantly down-regulated (P < .05) and hsa-miR-133a-3p was significantly up-regulated in patients with abnormal bone mass (P < .05). The expression of hsa-miR-182-5p was not substantially different from that of the osteoporosis group. Further, one-factor analysis showed that hsa-miR-21-5p, hsa-miR-133a-3p, age, and BMI were the influencing factors of primary osteoporosis (P < .05) and osteopenia was negatively correlated with the expression level of hsa-miR-21-5p and BMI (P < .05). We found through bioinformatics analysis that the Hub genes of hsa-miR-21-5p and hsa-miR-133a-3p are associated with bone homeostasis imbalance. Hsa-miR-21-5p and hsa-miR-133a-3p are significantly changed in the plasma of primary osteoporosis and osteopenia patients. The relative expression levels of miRNA21 and miRNA133a are correlated with bone mineral density levels. A large sample size study is required to validate its potential value as a clinical indicator.
本研究调查了人血浆中循环的hsa-miR-21-5p、hsa-miR-133a-3p、hsa-miR-182-5p与骨密度水平之间的相关性。共招募了120名骨量正常、骨量减少和骨质疏松的受试者。采用RT-PCR检测患者血浆中hsa-miR-21-5p、hsa-miR-133a-3p、hsa-miR-182-5p的相对表达水平，并结合体格检查数据进行相关性分析。使用生物信息学工具分析hsa-miR-21-5p和hsa-miR-133a-3p对骨代谢的潜在影响。与正常组相比，骨量异常患者血浆中hsa-miR-21-5p表达显著下调（P<0.05），hsa-miR-133a-3p表达显著上调（P<0.05）。hsa-miR-182-5p的表达与骨质疏松组相比无明显差异。此外，单因素分析显示hsa-miR-21-5p、hsa-miR-133a-3p、年龄和BMI是原发性骨质疏松症的影响因素（P<0.05），骨量减少与hsa-miR-21-5p和BMI的表达水平呈负相关（P<0.05）。我们通过生物信息学分析发现，hsa-miR-21-5p和hsa-miR-133a-3p的Hub基因与骨稳态失衡有关。原发性骨质疏松症和骨量减少患者血浆中hsa-miR-21-5p和hsa-miR-133a-3p显著变化。miRNA21和miRNA133a的相对表达水平与骨密度水平相关。需要进行大样本量研究以验证其作为临床指标的潜在价值。
