Karimi Parastoo, Mirzaei Mohammad Reza, Arami Farzaneh, Bagheri-Hosseinabadi Zahra, Abbasifard Mitra, Mohammadi-Hosseinabad Saeed, Jalali Zahra, Mahmoodi Mehdi, Hajizadeh Mohammad Reza
Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
BMC Musculoskelet Disord. 2025 Aug 9;26(1):772. doi: 10.1186/s12891-025-08970-9.
Zinc (Zn) levels are reportedly lower in osteoporosis patients and may be linked to bone health. MicroRNAs (miRNAs) have also been implicated in the regulation of bone remodeling. This study aims to investigate the role of selected miRNAs and their potential interplay with Zn in the pathogenesis of osteoporosis.
In this case-control study, 50 patients with osteoporosis and 50 healthy controls were recruited. The serum transcript levels of miRNAs were assessed using Real-time PCR.
Levels of Zn was significantly (P = 0.0047) lower in the serum samples from osteoporosis patients in comparison to the healthy controls. The transcript level of miR-34a-5p (Fold change = 2.59, P = 0.008) and miR-335-5p (Fold change = 1.90, P = 0.013) was upregulated significantly in the serum samples of patients with osteoporosis compared to that of the healthy controls. Zn level in osteoporosis patients had significant negative correlation with transcript levels of miR-335-5p (r= -0.24, P = 0.037). Zn level had positive correlation with Z and T-scores and BMD of hip, spine, and femur. A negative correlation (r= -0.28 P = 0.044) was detected between transcript level of miR-34a-5p and femur BMD. There was a significant negative correlation between transcript level of miR-335-5p and hip BMD (r= -0.19 P = 0.038). miR-34a-5p and miR-335-5p showed diagnostic potential.
The findings suggest a potential interplay between Zn and miRNAs in the regulation of bone metabolism in osteoporosis patients. Zn and miRNAs may serve as biomarkers for bone health and a therapeutic target in osteoporosis management.
据报道，骨质疏松症患者体内的锌（Zn）水平较低，且可能与骨骼健康有关。微小RNA（miRNA）也参与了骨重塑的调节。本研究旨在探讨特定miRNA的作用及其与锌在骨质疏松症发病机制中的潜在相互作用。
在这项病例对照研究中，招募了50例骨质疏松症患者和50名健康对照者。使用实时定量PCR评估miRNA的血清转录水平。
与健康对照相比，骨质疏松症患者血清样本中的锌水平显著降低（P = 0.0047）。与健康对照相比，骨质疏松症患者血清样本中miR-34a-5p（倍数变化 = 2.59，P = 0.008）和miR-335-5p（倍数变化 = 1.90，P = 0.013）的转录水平显著上调。骨质疏松症患者的锌水平与miR-335-5p的转录水平呈显著负相关（r = -0.24，P = 0.037）。锌水平与髋部、脊柱和股骨的Z值、T值及骨密度呈正相关。miR-34a-5p的转录水平与股骨骨密度呈负相关（r = -0.28，P = 0.044）。miR-335-5p的转录水平与髋部骨密度呈显著负相关（r = -0.19，P = 0.038）。miR-34a-5p和miR-335-5p具有诊断潜力。
研究结果表明，锌与miRNA在骨质疏松症患者骨代谢调节中存在潜在的相互作用。锌和miRNA可能作为骨骼健康的生物标志物以及骨质疏松症治疗的靶点。
