Permogorov V I, Tiaglov B V
Mol Biol (Mosk). 1978 Sep-Oct;12(5):1050-6.
The effect of gene 5 protein from bacteriophage f1 on melting of double-stranded polynucleotides and DNAs has been investigated using the UV-spectroscopy method. A dependence of the melting temperature of polynucleotide (DNA)-gene 5 protein complexes upon the polynucleotide (DNA) GC-pair content has been detected. Using experimental data and examining some model systems we came to the supposition that the lowering of melting temperature of polynucleotide (DNA) induced by this protein is probably stipulated by intercalation of the protein tyrosyl residues into one of the chains of polynucleotide (DNA) double helix.
利用紫外光谱法研究了噬菌体f1的基因5蛋白对双链多核苷酸和DNA解链的影响。已检测到多核苷酸（DNA）-基因5蛋白复合物的解链温度对多核苷酸（DNA）中GC碱基对含量的依赖性。通过实验数据并研究一些模型系统，我们得出这样的推测：该蛋白诱导的多核苷酸（DNA）解链温度降低可能是由于蛋白的酪氨酰残基插入到多核苷酸（DNA）双螺旋的一条链中所致。
