Zhang Zhen, Jiang Xi, Zhang Li, Feng Ying, Tang Jiahao, Xu Yuan, Guo Yunchong, Yun Peng, Li Fangping
Department of Endocrinology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.
Department of Clinical Laboratory, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.
J Diabetes Res. 2025 Aug 5;2025:4051518. doi: 10.1155/jdr/4051518. eCollection 2025.
This mediation analysis was aimed at examining whether ghrelin mediates the association between gut microbiota and glucose metabolic indices. Fifty-five patients with newly diagnosed Type 2 diabetes mellitus (T2DM) and 40 healthy controls (HCs) were included. Serum ghrelin levels were measured by ELISA. Fecal 16S rRNA gene sequencing was performed, and gut microbiota differences were assessed using linear discriminant analysis effect size (LEfSe). The relative abundance values of differential bacteria were extracted to correlate with ghrelin and glucose metabolic indices. Mediation analysis was conducted to evaluate ghrelin's effect on the relationship between bacterial abundance and glucose metabolic indices. Acylated ghrelin (AG) levels were significantly elevated in the T2DM group and showed positive correlations with glycated hemoglobin (HbA1c), fasting blood glucose (FBG), C-peptide (CP), and homeostasis model assessment of insulin resistance (HOMA-IR). Microbial analysis revealed decreased abundances of , , and , but increased abundances of , , , , , and in T2DM. AG levels were negatively correlated with abundance. HbA1c and FBG showed positive correlations with , , and abundances, but negative correlations with , , and abundances. HOMA-IR was positively correlated with , , and abundances, but negatively correlated with and abundances. Mediation analysis demonstrated that serum AG partially mediated the association between abundance and both HOMA-IR (26.15%) and HbA1c (27.91%). AG mediated the relationship between abundance and HOMA-IR as well as HbA1c.
本中介分析旨在检验胃饥饿素是否介导肠道微生物群与糖代谢指标之间的关联。纳入了55例新诊断的2型糖尿病（T2DM）患者和40例健康对照（HC）。采用酶联免疫吸附测定法（ELISA）检测血清胃饥饿素水平。进行粪便16S rRNA基因测序，并使用线性判别分析效应大小（LEfSe）评估肠道微生物群差异。提取差异细菌的相对丰度值，以与胃饥饿素和糖代谢指标进行相关性分析。进行中介分析以评估胃饥饿素对细菌丰度与糖代谢指标之间关系的影响。T2DM组中酰化胃饥饿素（AG）水平显著升高，且与糖化血红蛋白（HbA1c）、空腹血糖（FBG）、C肽（CP）和胰岛素抵抗稳态模型评估（HOMA-IR）呈正相关。微生物分析显示，T2DM患者中 、 和 的丰度降低，但 、 、 、 、 和 的丰度增加。AG水平与 丰度呈负相关。HbA1c和FBG与 、 和 的丰度呈正相关，但与 、 和 的丰度呈负相关。HOMA-IR与 、 和 的丰度呈正相关，但与 和 的丰度呈负相关。中介分析表明，血清AG部分介导了 丰度与HOMA-IR（26.15%）和HbA1c（27.91%）之间的关联。AG介导了 丰度与HOMA-IR以及HbA1c之间的关系。
