Madruga Liszt Y C, Baghersad Somayeh, da Câmara Paulo C F, Sabino Roberta M, Kipper Matt J, Popat Ketul C
Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80526, USA.
Department of Bioengineering, George Mason University, Fairfax, VA 22030, USA.
Res Sq. 2025 Aug 6:rs.3.rs-7014360. doi: 10.21203/rs.3.rs-7014360/v1.
This research introduces carboxymethyl kappa-carrageenan-chitosan polyelectrolyte multilayers as a promising and sustainable alternative to heparin, used in surface treatments for blood-contacting medical devices. The polysaccharide-based surface coatings have good cytocompatibility and resist microbial adhesion of both Pseudomonas aeruginosa and Staphylococcus aureus. The blood compatibility of surfaces containing carboxymethyl-kappa-carrageenan was directly compared to similar polyelectrolyte multilayers containing heparin. The carboxymethyl-kappa-carrageenan surfaces inhibit whole-blood clotting like heparin-containing surfaces. Blood clotting is mediated by both protein-surface and cell-surface interactions. The carboxymethyl-kappa-carrageenan surfaces adsorb more fibrinogen and less albumin than the heparin surfaces, and they also exhibit reduced platelet and leukocyte adhesion, compared to heparin surfaces. These results suggest that carboxymethyl-kappa-carrageenan may represent a sustainable alternative to heparin as an anticoagulant surface modification.
本研究引入了羧甲基κ-卡拉胶-壳聚糖聚电解质多层膜，作为肝素的一种有前景的可持续替代品，用于与血液接触的医疗器械的表面处理。基于多糖的表面涂层具有良好的细胞相容性，并且能够抵抗铜绿假单胞菌和金黄色葡萄球菌的微生物粘附。将含有羧甲基-κ-卡拉胶的表面的血液相容性与含有肝素的类似聚电解质多层膜直接进行比较。羧甲基-κ-卡拉胶表面像含肝素表面一样抑制全血凝固。血液凝固是由蛋白质-表面和细胞-表面相互作用介导的。与肝素表面相比，羧甲基-κ-卡拉胶表面吸附更多的纤维蛋白原和更少的白蛋白，并且它们还表现出更低的血小板和白细胞粘附。这些结果表明，羧甲基-κ-卡拉胶可能作为一种抗凝血表面修饰，成为肝素的可持续替代品。
