Zhou Jinyu, Lian Shuai, Geng Zijian, Yang Yuejie, Wu Rui, Wang Jianfa
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No.5 Xinfeng Road, Daqing, 163319, P. R. China.
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affairs, Daqing, 163319, China.
In Vitro Cell Dev Biol Anim. 2025 Aug 13. doi: 10.1007/s11626-025-01055-w.
Probiotics can support the immune function of dairy cows and contribute to the synthesis of milk components in mammary gland tissue. Bovine lactoferrin (bLF) possesses immune-regulating and nutritional properties; however, the impact of probiotics on bLF remains unclear. This study aimed to investigate whether probiotics can enhance the synthesis and secretion of bLF in the mammary gland, with a particular focus on the specific mechanisms by which Lactiplantibacillus plantarum (L. plantarum) regulates bLF. Primary bovine mammary epithelial cells (BMECs) were cultured in six-well plates and treated with various types of probiotics. The expression of bLF was evaluated using quantitative real-time PCR (qRT-PCR), Western blot, and enzyme-linked immunosorbent assay (ELISA). The expression of transcription factors associated with the bLF promoter region, specifically, was analyzed through qRT-PCR and Western blot. Lacticaseibacillus rhamnosus (L. rhamnosus), Streptococcus thermophilus (S. thermophilus), Bifidobacterium (Bifido.), and L. plantarum upregulated bLF gene and protein expression to varying extents, with L. plantarum exhibiting the most pronounced effect. Furthermore, L. plantarum was found to regulate the expression of phosphorylated STAT3 and AP-1. These findings indicate that probiotics can influence the expression of bLF in mammary gland tissue. Additionally, L. plantarum modulates the production of bLF via the STAT3 and AP-1 transcription factor pathways.
益生菌可以支持奶牛的免疫功能，并有助于乳腺组织中乳成分的合成。牛乳铁蛋白（bLF）具有免疫调节和营养特性；然而，益生菌对bLF的影响仍不清楚。本研究旨在探讨益生菌是否能增强乳腺中bLF的合成和分泌，特别关注植物乳杆菌（L. plantarum）调节bLF的具体机制。将原代牛乳腺上皮细胞（BMECs）培养在六孔板中，并用各种类型的益生菌进行处理。使用定量实时PCR（qRT-PCR）、蛋白质免疫印迹法和酶联免疫吸附测定（ELISA）评估bLF的表达。通过qRT-PCR和蛋白质免疫印迹法分析与bLF启动子区域相关的转录因子的表达。鼠李糖乳杆菌（L. rhamnosus）、嗜热链球菌（S. thermophilus）、双歧杆菌（Bifido.）和植物乳杆菌均不同程度地上调了bLF基因和蛋白质的表达，其中植物乳杆菌的作用最为显著。此外，发现植物乳杆菌可调节磷酸化STAT3和AP-1的表达。这些发现表明，益生菌可以影响乳腺组织中bLF的表达。此外，植物乳杆菌通过STAT3和AP-1转录因子途径调节bLF的产生。
