组胺对培养的新生大鼠心脏细胞的变时效应。

Chronotropic effect of histamine on cultured neonatal rat heart cells.

作者信息

Csete K, Auclair M C, Lechat P

出版信息

Eur J Pharmacol. 1979 Sep 15;58(2):117-23. doi: 10.1016/0014-2999(79)90002-5.

Abstract

The positive chronotropic effect (PCE) of histamine in cultured neonatal rat heart cells was monitored using a microscopic method as well as an electro-optically recording device. The action potential frequency was also measured (by means of microelectrodes). An increase in PCE was noted when histamine (from 1 X 10(-6) M to 1 X 10(-5) M) was added to the cells. However, higher concentrations (from 1 X 10(-5) M to 1 X 10(-4) M) were less effective. The PCE of histamine was reduced by pretreating the cells with antihistaminic drugs. H1-blocking agents (promethazine and mepyramine) were more potent than H2-blocking drugs (metiamide and cimetidine). In addition, the PCE of histamine was abolished when the cells were in presence of high K+ medium (26 mEq) but contraction and action potential amplitudes were increased. Our results demonstrate that these cultures respond to histamine and that this response is abolished by antihistaminic drugs thus suggesting the H1 and/or H2 receptors may be present in the neonatal rat heart cell cultures.

摘要

使用显微镜方法以及电光记录装置监测组胺对培养的新生大鼠心脏细胞的正性变时作用（PCE）。还测量了动作电位频率（通过微电极）。当向细胞中加入组胺（从1×10⁻⁶ M至1×10⁻⁵ M）时，观察到PCE增加。然而，更高浓度（从1×10⁻⁵ M至1×10⁻⁴ M）的效果较差。用抗组胺药物预处理细胞可降低组胺的PCE。H1受体阻断剂（异丙嗪和美吡拉敏）比H2受体阻断药物（甲硫米特和西咪替丁）更有效。此外，当细胞处于高钾培养基（26 mEq）中时，组胺的PCE消失，但收缩和动作电位幅度增加。我们的结果表明，这些培养物对组胺有反应，并且这种反应被抗组胺药物消除，因此表明新生大鼠心脏细胞培养物中可能存在H1和/或H2受体。