Valen G, Skjelbakken T, Vaage J
Department of Surgery, University of Tromsø, Norway, Sweden.
Mol Cell Biochem. 1995 May 10;146(1):55-61. doi: 10.1007/BF00926882.
The role of histamine in cardiac physiology and pathophysiology is not clarified, but is dependent on species. The effects of exogenous histamine in Langendorff-perfused rat hearts were investigated. 1 mM, 100, 10, 1 and 0.1 microM of histamine (n = 7 each) as 15 min infusions were employed in a dose-response study, and compared to control perfused hearts (n = 7). In another experimental series, 100 microM histamine (n = 15) was added during reperfusion after 25 min global ischemia, and compared to control ischemia-reperfusion (n = 15). The maximal response to histamine in the dose-response study (100 microM) was an increase of left ventricular developed pressure to 126 +/- 8% of initial value (mean +/- SEM, p < 0.04), and increase of coronary flow to 152+6% (p < 0.02) after 5 min infusion. 100 microM histamine did not significantly influence heart rate or rhythm. The lowest concentration (0.1 microM) did not have effects cardiac performance. Reperfusion with histamine for 2 min after ischemia reduced left ventricular developed pressure to 68 +/- 10% of initial value versus 116+17% in ischemic controls (p < 0.05), and increased left ventricular end-diastolic pressure to 24 +/- 8 mmHg compared to 6 +/- 2 mmHg in controls (p < 0.04). Left ventricular pressures were similar in hearts reperfused with histamine and in ischemic controls for the rest of the observation. Coronary flow increased during reperfusion in hearts given histamine. Histamine had a dose-dependent positive inotropic and vasodilatory effect in isolated rat hearts. Exogenous histamine had only minor effects on post-ischemic cardiac function.
组胺在心脏生理学和病理生理学中的作用尚不清楚，且因物种而异。研究了外源性组胺对Langendorff灌注大鼠心脏的影响。在剂量反应研究中，以1 mM、100、10、1和0.1 μM的组胺（每组n = 7）进行15分钟的输注，并与对照灌注心脏（n = 7）进行比较。在另一个实验系列中，在25分钟全心缺血后的再灌注期间加入100 μM组胺（n = 15），并与对照缺血-再灌注组（n = 15）进行比较。剂量反应研究中对组胺的最大反应（100 μM）是在输注5分钟后左心室舒张末压增加至初始值的126±8%（平均值±标准误，p < 0.04），冠状动脉血流量增加至152 + 6%（p < 0.02）。100 μM组胺对心率或心律无显著影响。最低浓度（0.1 μM）对心脏功能无影响。缺血后用组胺再灌注2分钟可使左心室舒张末压降至初始值的68±10%，而缺血对照组为116 + 17%（p < 0.05），与对照组的6±2 mmHg相比，左心室舒张末压增加至24±8 mmHg（p < 0.04）。在其余观察期间，用组胺再灌注的心脏和缺血对照组的左心室压力相似。给予组胺的心脏在再灌注期间冠状动脉血流量增加。组胺对离体大鼠心脏具有剂量依赖性的正性肌力和血管舒张作用。外源性组胺对缺血后心脏功能的影响较小。
