Microbiome of the Proximal Small Intestine in Patients with Acute Pancreatitis.

Currently, due to the complexity of obtaining samples, specific features of laboratory processing and analysis of the results, there is a lack of data on the microbial signature of the small intestine in healthy and diseased states of the upper gastrointestinal tract. To investigate the characteristics of the small intestinal microbiome in acute pancreatitis of varying severity and to identify correlations with clinical factors. This study included 30 patients with acute pancreatitis of varying severity treated between 1 January 2019 and 31 December 2021. The composition of the microbiota was analyzed by metagenomic sequencing of the 16S rRNA gene from jejunal samples. The mortality rate in the study group was 23.3%. The small intestinal microbiome was dominated by (median relative abundance 19.2%, interquartile range 6.4-35.1%), (3.4%; 0.6-7%), (2.7%; 0.6-5%), (2.2%; 0.3-5.9%), (1.5%; 0.3-8%), (0.9%; 0.2-10%), (0.8%; 0.2-4.3%), and (0.2%; 0.1-0.9%). More severe disease was associated with decreased abundance of and . In contrast, the relative abundance of the genera (species //), Actinomyces, and several genera within the family Enterobacteriaceae was higher in these patients. The state of the microbiota has important prognostic value and correlates with the duration from the onset of the pain syndrome to the time of receiving qualified care in the hospital.