[物种名称]成熟性腺的从头组装、特征分析及比较转录组分析

De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Gonads in .

作者信息

Zhou Yicheng, Liang Weiqian, Wang Kaifeng, Zheng Peng, Lin Shengyue, Yang Haiying, Cai Guojun, Deng Ziyan, Han Chong, Li Qiang

机构信息

School of Life Sciences, Guangzhou University, Guangzhou 510006, China.

出版信息

Animals (Basel). 2025 Jul 24;15(15):2184. doi: 10.3390/ani15152184.

DOI:10.3390/ani15152184

PMID:40804974

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12345467/

Abstract

is a significant aquatic fish in South China, renowned for its tasty meat. Nonetheless, related studies are deficient concerning the gonadal development of . This paper presents the first comparative transcriptome analysis of the gonads of female and male . A total of 84,886 unigenes were assembled, with 42,322 effectively annotated to the Nr, SwissProt, KEGG, KOG, and GO databases. Furthermore, comparative transcriptomic analysis of was conducted to examine its gonadal development. A total of 14,972 differentially expressed genes (DEGs) were discovered. In the testis, the expression of 11,928 unigenes was significantly upregulated, while 3044 were significantly downregulated. Numerous DEGs associated with steroidogenesis, gonadal differentiation and development, and gametogenesis in teleost fish were identified. The results provide empirical support for further study of genes and pathways associated with sex determination and gonadal differentiation in teleost fish.

摘要

是中国南方一种重要的水生鱼类，以其美味的鱼肉而闻名。然而，关于其性腺发育的相关研究却很缺乏。本文首次对雌性和雄性的性腺进行了比较转录组分析。共组装了84,886个单基因，其中42,322个有效地注释到Nr、SwissProt、KEGG、KOG和GO数据库。此外，对进行了比较转录组分析以研究其性腺发育。共发现了14,972个差异表达基因（DEG）。在睾丸中，11,928个单基因的表达显著上调，而3044个显著下调。鉴定出了许多与硬骨鱼类固醇生成、性腺分化和发育以及配子发生相关的DEG。这些结果为进一步研究硬骨鱼性别决定和性腺分化相关的基因和途径提供了实证支持。