State Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou, 510275, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266373, China.
School of Life Sciences, Guangzhou University, Guangzhou 510006, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Sep;47:101115. doi: 10.1016/j.cbd.2023.101115. Epub 2023 Aug 5.
The wild Coreoperca whiteheadi is considered as the primordial species in sinipercine fish, which has valuable genetic information. Unfortunately, C. whiteheadi was listed as a near-threatened species because of the environmental pollution, over-exploitation and species invasion. Therefore, more genetic information is needed to have a better understanding of gonadal development in C. whiteheadi. Here, the first gonadal transcriptomes analysis of C. whiteheadi was conducted and 277.14 million clean reads were generated. A total of 96,753 unigenes were successfully annotated. By comparing ovary and testis transcriptomes, a total of 21,741 differentially expressed genes (DEGs) were identified, of which 12,057 were upregulated and 9684 were downregulated in testes. Among them, we also identified about 53 differentially expressed sex-biased genes. Subsequently, the expression of twenty-four DEGs were confirmed by real-time fluorescence quantitative PCR. Furthermore, the histological analysis was conducted on ovaries and testes of one-year-old C. whiteheadi. Our results provided basic support for further studies on the function of sex-biased genes and the molecular mechanism of sex determination and reproduction in C. whiteheadi.
野生白头鱥被认为是鱥科鱼类的原始物种，具有有价值的遗传信息。不幸的是，由于环境污染、过度捕捞和物种入侵，白头鱥被列为近危物种。因此，需要更多的遗传信息来更好地了解白头鱥的性腺发育。本研究首次对白头鱥的性腺转录组进行了分析，共产生了 2.7714 亿条清洁reads，成功注释了 96753 条unigenes。通过比较卵巢和精巢转录组，共鉴定出 21741 个差异表达基因（DEGs），其中 12057 个在精巢中上调，9684 个在精巢中下调。其中，我们还鉴定出约 53 个差异表达的性别偏向基因。随后，通过实时荧光定量 PCR 验证了 24 个 DEGs 的表达情况。此外，对一年龄白头鱥的卵巢和精巢进行了组织学分析。我们的研究结果为进一步研究白头鱥中性别偏向基因的功能以及性别决定和繁殖的分子机制提供了基础支持。
