Aluai-Cunha Catarina, Oliveira Diana, Gregório Hugo, Petrucci Gonçalo, Correia Alexandra, Serra Cláudia, Santos Andreia
Department of Veterinary Clinics, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, R. Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Animal Science and Study Centre (CECA) 4050-313 Porto, Portugal.
Animals (Basel). 2025 Jul 27;15(15):2208. doi: 10.3390/ani15152208.
Mast cell tumours (MCT) are the most common cutaneous neoplasms in dogs, with variable behaviours and patient survival time. Both indolent and aggressive forms have been described, but much remains to be explored regarding prognosis and therapy. Evidence has highlighted the influence of microbiota on multiple health and disease processes, including certain types of cancer in humans. However, knowledge remains scarce regarding microbiota biology and its interactions in both humans and canine cancer patients. This study aimed to characterise the faecal microbiota of dogs with MCT and compare it with that of healthy individuals. Twenty-eight dogs diagnosed with MCT and twenty-eight healthy dogs were enrolled in the study. Faecal samples were collected and analysed by Illumina sequencing of 16S rRNA genes. Alpha diversity was significantly lower in dogs with cancer, and the species diversity InvSimpson Indexwas reduced ( = 0.019). Principal coordinate analysis showed significant differences in the bacterial profile of the two groups: there was a significant lower abundance of the genera , , , and and, conversely, a significant increase in the genera and in diseased dogs. At the phylum level, was significantly reduced in diseased dogs (25% in controls vs. 19% in MCT dogs). In conclusion, sequencing analysis provided an overview of the bacterial profile and showed statistical differences in the microbial communities of dogs with MCT compared with healthy dogs, suggesting a link between the gut microbiota and MCT in this species.
肥大细胞瘤（MCT）是犬类最常见的皮肤肿瘤，其行为和患者存活时间各不相同。已描述了惰性和侵袭性两种形式，但在预后和治疗方面仍有许多有待探索之处。有证据强调了微生物群对多种健康和疾病过程的影响，包括人类的某些类型癌症。然而，关于微生物群生物学及其在人类和犬类癌症患者中的相互作用，知识仍然匮乏。本研究旨在对患有MCT的犬类粪便微生物群进行特征分析，并将其与健康个体的粪便微生物群进行比较。28只被诊断患有MCT的犬和28只健康犬被纳入研究。收集粪便样本并通过16S rRNA基因的Illumina测序进行分析。癌症犬的α多样性显著降低，物种多样性逆辛普森指数降低（P = 0.019）。主坐标分析显示两组细菌谱存在显著差异：患病犬中，、、和属的丰度显著降低，相反，患病犬中属和属的丰度显著增加。在门水平上，患病犬中的显著降低（对照组为25%，MCT犬为19%）。总之，测序分析提供了细菌谱的概述，并显示患有MCT的犬与健康犬的微生物群落存在统计学差异，表明该物种的肠道微生物群与MCT之间存在联系。
