Clinical Laboratory, Guangzhou Cadre Health Management Center, Guangzhou No. 11 People's Hospital, Guangzhou, China.
Clinical Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China.
Sci Rep. 2023 May 13;13(1):7797. doi: 10.1038/s41598-023-34765-w.
Fecal samples from participants aged 60-80 were collected and sequenced by a high-throughput second-generation sequencer to explore the structural composition of gut microbiota in elderly patients with hepatocellular carcinoma(HCC). Comparison of gut microbiota between patients with hepatocellular carcinoma and healthy controls, α diversity and β diversity were statistically different. At the genus level, compared with the normal group, the abundance of A Blautia, Fusicatenibacter, Anaerostipes, Lachnospiraceae_ND3007_group, CAG-56, Eggerthella, Lachnospiraceae_FCS020_group and Olsenella were decreased significantly in the LC group. In contrast, the abundance of Escherichia-Shigella, Fusobacterium, Megasphaera, Veillonella, Tyzzerella_4, Prevotella_2 and Cronobacter increased significantly. The KEGG and COG pathway analyses showed that the dysbiosis of gut bacteria in primary liver carcinoma is associated with several pathways, including amino acid metabolism, replication and repair, nucleotide metabolism, cell motility, cell growth and death, and transcription. Age is negatively associated with the abundance of Bifidobacterium. Lachnospiraceae_ ND3007_ group, [Eubacterium]hallii_group, Blautia, Fuscatenibacter and Anaerostipes are negatively correlated with ALT, AST and GGT levels (p < 0.05), respectively. Alpha-fetoprotein (AFP) is positively associated with the abundance of Erysipelatoclostridium, Magasphaera, Prevotella 2, Escherichia-Shigella, Streptococcus and [Eubacterium]eligens_group (p < 0.05), respectively. A random forest model showed that the genera Eggerthella, Anaerostipes, and Lachnospiraceae ND3007 group demonstrated the best predictive capacity. The area under the Receiver Operating Characteristic Curve of Eggerthella, Anaerostipes and Lachnospiraceae_ ND3007_ group are 0.791, 0.766 and 0.730, respectively. These data are derived from the first known gut microbiome study in elderly patients with hepatocellular carcinoma. Potentially, specific microbiota can be used as a characteristic index for screening, diagnosis, and prognosis of gut microbiota changes in elderly patients with hepatocellular carcinoma and even as a therapeutic clinical target.
从年龄在 60-80 岁的参与者中收集粪便样本，并使用高通量第二代测序仪对其进行测序，以探索老年肝细胞癌（HCC）患者肠道微生物组的结构组成。对 HCC 患者和健康对照组的肠道微生物组进行比较，α多样性和β多样性有统计学差异。在属水平上，与正常组相比，LC 组中 A Blautia、Fusicatenibacter、Anaerostipes、Lachnospiraceae_ND3007_group、CAG-56、Eggerthella、Lachnospiraceae_FCS020_group 和 Olsenella 的丰度显著降低。相反，Escherichia-Shigella、Fusobacterium、Megasphaera、Veillonella、Tyzzerella_4、Prevotella_2 和 Cronobacter 的丰度显著增加。KEGG 和 COG 途径分析表明，原发性肝癌肠道细菌的失调与多种途径有关，包括氨基酸代谢、复制和修复、核苷酸代谢、细胞运动、细胞生长和死亡以及转录。年龄与双歧杆菌的丰度呈负相关。Lachnospiraceae_ND3007_group、[Eubacterium]_hallii_group、Blautia、Fuscatenibacter 和 Anaerostipes 分别与 ALT、AST 和 GGT 水平呈负相关（p<0.05）。甲胎蛋白（AFP）与 Erysipelatoclostridium、Magasphaera、Prevotella 2、Escherichia-Shigella、Streptococcus 和 [Eubacterium]_eligens_group 的丰度呈正相关（p<0.05）。随机森林模型显示，Eggerthella、Anaerostipes 和 Lachnospiraceae_ND3007_group 这三个属具有最佳的预测能力。Eggerthella、Anaerostipes 和 Lachnospiraceae_ND3007_group 的受试者工作特征曲线下面积分别为 0.791、0.766 和 0.730。这些数据来源于对老年肝细胞癌患者进行的首次肠道微生物组研究。潜在地，特定的微生物群可以作为老年肝细胞癌患者肠道微生物组变化筛查、诊断和预后的特征指标，甚至可以作为治疗的临床靶点。
Appl Microbiol Biotechnol. 2024-12
Hepatobiliary Pancreat Dis Int. 2017-8-15
Int J Biol Sci. 2019-9-7
J Gastroenterol Hepatol. 2022-10
Animals (Basel). 2025-7-27
Front Pharmacol. 2025-5-19
J Microbiol Biotechnol. 2025-5-15
Anticancer Res. 2023-2
Curr Opin Support Palliat Care. 2023-3-1
Front Microbiol. 2022-1-31
Zotero 插件
