Feline urinary tract infections (UTIs) present a common challenge in veterinary practice, underscoring the importance of understanding local bacterial pathogens and antimicrobial resistance (AMR). This study determined bacterial prevalence and antimicrobial susceptibility in cats at Kasetsart University's Veterinary Teaching Hospital in Bangkok, Thailand. Of the 543 cystocentesis urine samples collected from 428 cats, 115 (21.2%) tested positive for bacterial cultures, leading to a diagnosis of UTIs in 95 cats (22.2%). The most prevalent isolates included (24.8%), species (19.2%), (13.6%), (12.0%), and species (12.0%). (8.8%) and (5.6%) were the predominant species. Rare pathogens such as and were also identified. Antimicrobial testing revealed alarming resistance, with 69.2% of isolates exhibiting multidrug resistance (MDR). and showed high resistance to amoxicillin/clavulanic acid (AMC) (45.2-70.6%) and sulfamethoxazole/trimethoprim (SXT) (51.6-52.9%). exhibited 85.7% resistance to AMC. Methicillin resistance was identified in 41.7% of isolates, particularly high in (75.0%) and (71.4%). High fluoroquinolone resistance among MDR isolates further exacerbates AMR concerns. These results indicate that MDR Gram-negative, , and species complicate the empirical treatment of feline UTIs, highlighting significant implications for AMR in veterinary practice.