4',6-二脒基-2-苯基吲哚（DAPI）与双链核糖核酸的相互作用。
Interaction of DAPI with double-stranded ribonucleic acids.
作者信息
Manzini G, Xodo L, Barcellona M L, Quadrifoglio F
出版信息
Nucleic Acids Res. 1985 Dec 20;13(24):8955-67. doi: 10.1093/nar/13.24.8955.
Abstract
The interaction of DAPI with natural and synthetic double-stranded polyribonucleotides was studied with different optical and calorimetric methods. The results were similar to those obtained previously with double-stranded polydeoxynucleotides, i.e. two interaction modes, the first of which shows high affinity for AU clusters and consequent strong fluorescence enhancement. The results suggest caution in the use of DAPI as selective fluorescent staining agent for DNA in the presence of RNA. A narrow groove binding model with hydrogen bonds between DAPI and AU pairs is proposed. An intercalation mechanism can be excluded because of the non planarity of DAPI molecule.
摘要
采用不同的光学和量热法研究了4',6-二脒基-2-苯基吲哚（DAPI）与天然和合成双链聚核糖核苷酸的相互作用。结果与先前用双链聚脱氧核苷酸获得的结果相似，即存在两种相互作用模式，其中第一种模式对AU簇具有高亲和力，从而导致强烈的荧光增强。结果表明，在存在RNA的情况下，将DAPI用作DNA的选择性荧光染色剂时需谨慎。提出了一种在DAPI和AU碱基对之间形成氢键的窄沟结合模型。由于DAPI分子的非平面性，可以排除嵌入机制。
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