Xia Antong, Wu Yanyou, Zhai Kun, Xiang Dongshan, Li Lin, Qin Zhanghui, Twagirayezu Gratien
Hubei Key Laboratory of Selenium Resource Research and Biological Application, Hubei Minzu University, Enshi 445000, China.
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China.
Plants (Basel). 2025 Jul 29;14(15):2337. doi: 10.3390/plants14152337.
Although () is vital in agricultural production and vulnerable to the pathogen , the intracellular water-nutrient metabolism and immunoregulation of infection in leaves remain unclear. This study aimed to analyze the responsive mechanisms of -infected using rapid detection technology. Six soil groups planted with Yangtze No. 5 were inoculated with varying concentrations (from 0 to 10 × 10 spores/mL). The results showed that at the highest infection concentration (PWB5, 10 × 10 spores/mL) of leaves, the plant electrophysiological parameters showed the intracellular water-holding capacity (IWHC), the intracellular water use efficiency (IWUE), and the intracellular water translocation rate (IWTR) declined by 41.99-68.86%. The unit for translocation of nutrients (UNF) increased by 52.83%, whereas the nutrient translocation rate (NTR), the nutrient translocation capacity (NTC), the nutrient active translocation (NAT) value, and the nutrient active translocation capacity (NAC) decreased by 52.40-77.68%. The cellular energy metabolism decreased with worsening infection, in which the units for cellular energy metabolism (∆G) and cellular energy metabolism (∆G) of the leaves decreased by 44.21% and 78.14% in PWB5, respectively. Typically, based on distribution of B-type dielectric substance transfer percentage (BPn), we found PWB4 (8 × 10 spores/mL) was the maximal immune response concentration, as evidenced by a maximal BPn (B-type dielectric substance transfer percentage based on resistance), with increasing lignin and cork deposition to enhance immunity, and a minimum BPn (B-type dielectric substance transfer percentage based on capacitive reactance), with a decreasing quantity of surface proteins in the leaves. This study suggests plant electrophysiological parameters could characterize intracellular water-nutrient metabolism and immunoregulation of leaves under various infection concentrations, offering a dynamic detection method for agricultural disease management.
尽管（）在农业生产中至关重要且易受病原体侵害，但（）叶片中细胞内水分 - 养分代谢及感染免疫调节仍不清楚。本研究旨在利用快速检测技术分析（）感染（）的响应机制。六个种植长江5号（）的土壤组接种了不同浓度（从0至10×10孢子/毫升）的（）。结果表明，在（）叶片的最高感染浓度（PWB5，10×10孢子/毫升）下，植物电生理参数显示细胞内持水量（IWHC）、细胞内水分利用效率（IWUE）和细胞内水分转运速率（IWTR）下降了41.99 - 68.86%。养分转运单位（UNF）增加了52.83%，而养分转运速率（NTR）、养分转运能力（NTC）、养分主动转运（NAT）值和养分主动转运能力（NAC）下降了52.40 - 77.68%。随着（）感染加剧，细胞能量代谢下降，其中叶片的细胞能量代谢单位（∆G）和细胞能量代谢（∆G）在PWB5中分别下降了44.21%和78.14%。通常，基于B型介电物质转移百分比（BPn）的分布，我们发现PWB4（8×10孢子/毫升）是最大免疫反应浓度，表现为最大的BPn（基于电阻的B型介电物质转移百分比），同时木质素和栓质沉积增加以增强免疫力；以及最小的BPn（基于容抗的B型介电物质转移百分比），伴随着（）叶片表面蛋白数量减少。本研究表明植物电生理参数可表征不同（）感染浓度下（）叶片的细胞内水分 - 养分代谢及免疫调节，为农业病害管理提供了一种动态检测方法。
