Sareło Przemysław, Wiśniewska-Wrona Maria, Sikora Monika, Mielan Bartosz, Gerasymchuk Yuriy, Wędzyńska Anna, Boiko Vitalii, Hreniak Dariusz, Szymonowicz Maria, Sobieszczańska Beata, Wawrzyńska Magdalena
Pre-Clinical Research Center, Wrocław Medical University, Karola Marcinkowskiego 1, 50-368 Wrocław, Poland.
Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 27, 50-370 Wrocław, Poland.
Int J Mol Sci. 2025 Jul 31;26(15):7403. doi: 10.3390/ijms26157403.
Chronic infected wounds remain a major medical challenge, particularly in the context of increasing antibiotic resistance. The objective of this study was to develop and evaluate chitosan-based (CS) sponges enhanced with graphene oxide (GO) as potential antimicrobial wound dressings. The composite sponges were fabricated using microcrystalline CS (MKCh) and 5% (/) GO, followed by freeze-drying and γ-sterilization (25 kGy). Physico-mechanical characterization showed that GO incorporation did not significantly alter tensile strength, while absorption and sorption capacities were improved, especially after sterilization. Structural and spectroscopic analyses confirmed increased porosity and molecular interaction between CS and GO. Cytocompatibility was verified in vitro using L-929 fibroblasts, with no cytotoxic effects observed in indirect contact. Antimicrobial activity tests demonstrated that GO-modified dressings exhibited enhanced activity against and , though results were strain-dependent and not uniformly superior to CS alone. Notably, antifungal efficacy against was reduced with GO addition. Overall, the developed GO-enriched CS sponges present favorable biocompatibility, mechanical resilience, and selective antimicrobial activity, supporting their potential application in chronic wound management. Further optimization of GO concentration and formulation is warranted to maximize antimicrobial efficacy across a broader spectrum of pathogens.
慢性感染伤口仍然是一个重大的医学挑战，尤其是在抗生素耐药性不断增加的背景下。本研究的目的是开发和评估以氧化石墨烯（GO）增强的壳聚糖基（CS）海绵作为潜在的抗菌伤口敷料。复合海绵采用微晶CS（MKCh）和5%（/）的GO制备，然后进行冷冻干燥和γ射线灭菌（25 kGy）。物理力学表征表明，加入GO不会显著改变拉伸强度，而吸收和吸附能力得到改善，尤其是在灭菌后。结构和光谱分析证实了孔隙率增加以及CS与GO之间的分子相互作用。使用L-929成纤维细胞在体外验证了细胞相容性，在间接接触中未观察到细胞毒性作用。抗菌活性测试表明，GO修饰的敷料对[具体细菌名称1]和[具体细菌名称2]表现出增强的活性，尽管结果因菌株而异，且并非始终优于单独的CS。值得注意的是，添加GO后对[具体真菌名称]的抗真菌效果降低。总体而言，所开发的富含GO的CS海绵具有良好的生物相容性、机械弹性和选择性抗菌活性，支持它们在慢性伤口管理中的潜在应用。有必要进一步优化GO浓度和配方，以在更广泛的病原体范围内最大化抗菌效果。
