Gospodarek Janina, Boligłowa Elżbieta, Gondek Krzysztof, Smoroń Krzysztof, Paśmionka Iwona B
Department of Microbiology and Biomonitoring, University of Agriculture, al. A. Mickiewicza 21, 31-120 Krakow, Poland.
Department of Agricultural and Environmental Chemistry, University of Agriculture, al. A. Mickiewicza 21, 31-120 Krakow, Poland.
Molecules. 2025 Aug 6;30(15):3300. doi: 10.3390/molecules30153300.
Combining products of natural origin with different mechanisms of action on insect herbivores may provide an alternative among methods of plant protection against pests that are less risky for the environment. The aim of the study was to evaluate the effectiveness of mixtures of L. essential oil and diatomite (EO + DE) compared to each substance separately in reducing economically important pests such as black bean aphid (BBA) Scop., Colorado potato beetle (CPB) Say., and pea leaf weevil (PLW) L. The effects on mortality (all pests) and foraging intensity (CPB and PLW) were tested. The improvement in effectiveness using a mixture of EO + DE versus single components against BBA was dose- and the developmental stage-dependent. The effect of enhancing CPB foraging inhibition through DE addition was obtained at a concentration of 0.2% EO (both females and males of CPB) and 0.5% EO (males) in no-choice experiments. In choice experiments, mixtures EO + DE with both 0.2% and 0.5% EO concentrations resulted in a significant reduction in CPB foraging. A significant strengthening effect of EO 0.5% through the addition of DE at a dose of 10% against PLW males was observed in the no-choice experiment, while, when the beetles had a choice, the synergistic effect of a mixture of EO 0.5% and DE 10% was also apparent in females. In conclusion, the use of DE mixtures with EO from appears to be a promising strategy. The results support the idea of not using doses of EO higher than 0.5%.
将具有不同作用机制的天然来源产品与昆虫食草动物相结合，可能为植物保护免受害虫侵害的方法提供一种对环境风险较小的替代方案。本研究的目的是评估薰衣草精油（EO）和硅藻土（DE）混合物与每种物质单独使用相比，在减少黑豆蚜（BBA）、科罗拉多马铃薯甲虫（CPB）和豌豆叶象鼻虫（PLW）等经济上重要的害虫方面的有效性。测试了对死亡率（所有害虫）和觅食强度（CPB和PLW）的影响。使用EO + DE混合物相对于单一成分对BBA的有效性提高取决于剂量和发育阶段。在无选择实验中，添加DE增强CPB觅食抑制的效果在EO浓度为0.2%（CPB的雌性和雄性）和0.5%（雄性）时获得。在选择实验中，EO浓度为0.2%和0.5%的EO + DE混合物导致CPB觅食显著减少。在无选择实验中，观察到通过添加10%的DE对PLW雄性有显著的增强0.5% EO的效果，而当甲虫有选择时，0.5% EO和10% DE混合物的协同作用在雌性中也很明显。总之，使用DE与EO的混合物似乎是一种有前途的策略。结果支持不使用高于0.5% EO剂量的观点。
