Ricós-Muñoz Neus, Maicas Sergi, Tortajada-Girbés Miguel, Pina-Pérez Maria Consuelo
Departamento Microbiologia y Ecologia, Universitat de València, 46100 Burjassot (Valencia), Spain.
Pediatric Pulmonology and Allergy Unit, Hospital Universitari i Politècnic La Fe, 46026 Valencia, Spain.
Foods. 2025 Jul 24;14(15):2589. doi: 10.3390/foods14152589.
Allergy is recognized as a public health problem with pandemic consequences and is estimated to affect more than 50% of Europeans in 2025. Prebiotic and probiotic food implementation has recently emerged as an alternative strategy to promote immunomodulatory beneficial effects in allergic patients. Among prebiotics, algae represent a niche of research with enormous possibilities. The present study aims to evaluate the in vitro prebiotic potential of fucoidan from , , and algae, to promote the growth of and GG as probiotic bacteria added to the formulation of a novel yogurt. Concentrations of fucoidan of 100 and 2000 µg/mL were added to reference growth media and kinetic growth curves for both microorganisms were fitted to the Gompertz equation. Optimized prebiotic conditions for fucoidan were selected to validate in vitro results by means of the formulation of a novel fermented prebiotic yogurt. Conventional yogurts (including and subs. bulgaricus) were formulated with the different fucoidans, and production batches were prepared for and . Increased and populations in 1.7-2.2 log cycles just after 48 h of in vitro exposure were detected in fucoidan supplemented yogurt. and fucoidans were the most effective ones (500 µg/mL) promoting probiotic growth in new formulated yogurts (during the complete shelf life of products, 28 days). Diet supplementation with fucoidan can be proposed as a strategy to modulate beneficial microbiota against allergy.
过敏被认为是一个具有大流行后果的公共卫生问题，据估计到2025年将影响超过50%的欧洲人。益生元和益生菌食品的应用最近已成为一种替代策略，以促进对过敏患者的免疫调节有益作用。在益生元中，藻类是一个具有巨大潜力的研究领域。本研究旨在评估来自[具体藻类名称1]、[具体藻类名称2]和[具体藻类名称3]藻类的岩藻依聚糖的体外益生元潜力，以促进作为益生菌添加到新型酸奶配方中的嗜酸乳杆菌和双歧杆菌GG的生长。将100和2000 µg/mL的岩藻依聚糖浓度添加到参考生长培养基中，并将两种微生物的动力学生长曲线拟合到Gompertz方程。选择岩藻依聚糖的优化益生元条件，通过制备新型发酵益生元酸奶来验证体外实验结果。用不同的岩藻依聚糖制备传统酸奶（包括[具体名称1]和保加利亚亚种），并为嗜酸乳杆菌和双歧杆菌GG制备生产批次。在添加岩藻依聚糖的酸奶中，体外暴露48小时后就检测到嗜酸乳杆菌和双歧杆菌种群增加了1.7 - 2.2个对数周期。[具体藻类名称1]和[具体藻类名称2]的岩藻依聚糖是在新配制的酸奶中促进益生菌生长最有效的（500 µg/mL）（在产品的整个保质期28天内）。可以提出补充岩藻依聚糖饮食作为调节有益微生物群以对抗过敏的一种策略。
