Manev H, Pericić D
Pharmacol Biochem Behav. 1985 Nov;23(5):697-700. doi: 10.1016/0091-3057(85)90057-7.
Ether stress (2 X 2 min within 15 min) and dexamethasone treatment (1 mg/kg IP; 1, 3 and 12 hours before sacrifice), the procedures supposed to increase the activity of glutamate decarboxylase (GAD) in the hypothalamus, fail to affect the concentration of GABA in the rat hypophysis. Five and/or ten minutes post-mortem an increased GABA level in the hypothalamus and cingulate cortex, and a decreased GABA concentration in the hypophysis was found. Three and four hours after the IP administration of 1-cycloserine (50 mg/kg) and 1-glutamic acid-gamma-hydrazide (160 mg/kg) respectively (both drugs are inhibitors of GABA catabolism) the concentration of GABA raised in all the regions examined. On the basis of studies in the whole gland it might be concluded that the concentration of GABA in the hypophysis depends more on GABA release from extrahypophysial tissue and GABA degradation in the hypophysis than on the extrahypophysial GABA synthesis. Also on the basis of post-mortem studies in the whole gland no indication for the appearance of GABA synthesis in hypophysis could be found.
乙醚应激（15分钟内2次，每次2分钟）和地塞米松处理（腹腔注射1mg/kg；处死前1、3和12小时），这些本应增加下丘脑谷氨酸脱羧酶（GAD）活性的程序，却未能影响大鼠垂体中γ-氨基丁酸（GABA）的浓度。死后5分钟和/或10分钟时，发现下丘脑和扣带回皮质中GABA水平升高，而垂体中GABA浓度降低。分别腹腔注射1-环丝氨酸（50mg/kg）和1-谷氨酸-γ-酰肼（160mg/kg）（两种药物均为GABA分解代谢抑制剂）3小时和4小时后，所有检测区域的GABA浓度均升高。基于对整个腺体的研究可以得出结论，垂体中GABA的浓度更多地取决于垂体外组织释放的GABA以及垂体中GABA的降解，而不是垂体外GABA的合成。同样基于对整个腺体的死后研究，未发现垂体中有GABA合成的迹象。
