Fan Nai-Chia, Hsu Fang-Min, Cheng Chi-Hui, Lin Jui-Che
Division of Nephrology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan 333423, Taiwan.
Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan 333323, Taiwan.
Polymers (Basel). 2025 Jul 22;17(15):2006. doi: 10.3390/polym17152006.
Healthcare-associated infection, mainly through medical device-associated infection, remains a critical issue in hospital care. Bacterial adhesion, proliferation, and biofilm formation on the device surface have been considered the foremost cause of medical device-associated infection. Different means have been explored to reduce microbial attachment and proliferation, including forming a bactericidal or microbial adhesion-resistant surface layer. Fear of limited bactericidal capability if the dead microbes remained adhered to the surface has withheld the widespread use of a bactericidal surface in medical devices if it was intended for long-term use. By contrast, constructing a microbial adhesion-resistant or antifouling surface, such as a surface with zwitterionic functionality, would be more feasible for devices intended to be used for the long term. Nevertheless, a sophisticated multi-step chemical reaction process would be needed. Instead, a simple immersion method that utilized a novel mussel-inspired catechol compound with zwitterionic sulfobetaine functionality, ZDS, was explored in this investigation for the surface modification of substrates with distinctively different surface characteristics, including titanium and polyvinyl chloride. Dopamine, NaIO oxidants, and chemicals that could affect ionic interactions (NaCl and polyethyleneimine) were added to the ZDS-containing immersion solution to compare their effects on modifying titanium and PVC substrates. Furthermore, a layer-by-layer immersion method, in which the substrate was first immersed in the no-ZDS-added dopamine-containing solution, followed by the ZDS-containing solution, was also attempted on the PVC substrate. By properly selecting the immersion solution formulation and additional NaIO oxidation modification, the antibacterial capability of ZDS-modified substrates can be optimized without causing cytotoxicity. The maximum antibacterial percentages against were 84.2% and 81.7% for the modified titanium and PVC substrate, respectively, and both modified surfaces did not show any cytotoxicity.
医疗保健相关感染，主要通过与医疗器械相关的感染，仍然是医院护理中的一个关键问题。细菌在器械表面的粘附、增殖和生物膜形成被认为是与医疗器械相关感染的首要原因。人们已经探索了不同的方法来减少微生物的附着和增殖，包括形成杀菌或抗微生物粘附的表面层。如果死微生物仍粘附在表面，担心杀菌能力有限，这使得杀菌表面在长期使用的医疗器械中未能广泛应用。相比之下，构建抗微生物粘附或防污表面，如具有两性离子功能的表面，对于打算长期使用的器械来说更可行。然而，这需要复杂的多步化学反应过程。相反，本研究探索了一种简单的浸泡方法，该方法利用一种具有两性离子磺基甜菜碱功能的新型贻贝启发的儿茶酚化合物ZDS，对具有明显不同表面特性的底物进行表面改性，包括钛和聚氯乙烯。将多巴胺、NaIO氧化剂以及可能影响离子相互作用的化学物质（NaCl和聚乙烯亚胺）添加到含ZDS的浸泡溶液中，以比较它们对改性钛和PVC底物的影响。此外，还在PVC底物上尝试了逐层浸泡方法，即先将底物浸泡在不含ZDS的含多巴胺溶液中，然后再浸泡在含ZDS的溶液中。通过适当选择浸泡溶液配方和额外的NaIO氧化改性，可以优化ZDS改性底物的抗菌能力而不产生细胞毒性。改性钛和PVC底物对[具体细菌]的最大抗菌率分别为84.2%和81.7%，且两种改性表面均未显示任何细胞毒性。
