荔枝核总黄酮对诱导性肝损伤和肝纤维化的抑制作用研究。

Investigation of the inhibitory effects of total flavonoids of litchi seed on -induced liver damage and fibrosis.

作者信息

Luo Liuchun, Wang Jilong, Cheng Qiuchen, Lu Zuochao, Lv Jiahui, Xiao Suyu, Tang Lili, He Shanshan, Liu Dengyu, Tang Zeli, Li Qing, Zhan Tingzheng

机构信息

Department of Parasitology, Guangxi Medical University, Nanning, China.

Department of Clinical Laboratory, Liuzhou People's Hospital, Liuzhou, China.

出版信息

Front Cell Infect Microbiol. 2025 Jul 30;15:1625004. doi: 10.3389/fcimb.2025.1625004. eCollection 2025.

DOI:10.3389/fcimb.2025.1625004

PMID:40809521

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12343252/

Abstract

BACKGROUND

(), which is prevalent in Asian countries, including China, Korea and Vietnam, is known to cause liver fibrosis, leading to various liver diseases and potentially fatal outcomes. Total flavonoids of litchi seed (TFL), a traditional Chinese medicine abundant in the Southern China, is known for its multiple pharmacological activities, including anti-fibrotic, anti-oxidative and hepato-protective properties. The present study explored the inhibitory effects of TFL on liver damage caused by infection in rats.

METHODS

In the animal experiment, female rats were infected with and treated with TFL. Serum biochemical indicators were measured each week. Pathological changes in the rat liver were evaluated by examining the appearance of liver, and by performing hematoxylin and eosin (H&E) and Masson's trichrome staining. Through immunofluorescence and immunohistochemistry, the number of activated hepatic stellate cells (HSCs) were counted and the collagen deposition area in these cells were measured. In the cell experiment, rat hepatic stellate cells (HSC-T6) were stimulated with TGF-β1 (10 ng/ml) and treated with different concentrations of TFL. The expression levels of HSCs activation markers α-smooth muscle actin (α-SMA), collagen I, and fibronectin were detected using quantitative real-time polymerase chain reaction (RT-qPCR). The protein levels of α-SMA and collagen I was detected by Western blot.

RESULTS

TFL improved liver function in -infected rats, as indicated by reduced levels of transaminases, bilirubin, and bile acids. TFL also alleviated pathological changes in liver tissues. TFL also reduced the number of activated HSCs and downregulated fibrosis-related markers (collagen I/III, α-SMA) in rat liver. In HSC-T6, TFL inhibited HSCs activation by reducing the mRNA levels of α-SMA, collagen I, and fibronectin, and reduced the protein level of α-SMA and collagen I.

CONCLUSIONS

TFL attenuated -induced liver fibrosis in rats. Our study provided experimental evidence for the development of novel anti-liver fibrosis drugs and offer new insights into the treatment of infections.

摘要

背景

（）在包括中国、韩国和越南在内的亚洲国家普遍存在，已知会导致肝纤维化，进而引发各种肝脏疾病并可能导致致命后果。荔枝核总黄酮（TFL）是中国南方盛产的一种传统中药，以其多种药理活性而闻名，包括抗纤维化、抗氧化和肝脏保护特性。本研究探讨了TFL对大鼠感染引起的肝损伤的抑制作用。

方法

在动物实验中，雌性大鼠感染（）并用TFL治疗。每周测量血清生化指标。通过检查肝脏外观以及进行苏木精和伊红（H&E）染色及Masson三色染色来评估大鼠肝脏的病理变化。通过免疫荧光和免疫组织化学，计数活化肝星状细胞（HSC）的数量并测量这些细胞中的胶原沉积面积。在细胞实验中，用转化生长因子-β1（10 ng/ml）刺激大鼠肝星状细胞（HSC-T6）并用不同浓度的TFL处理。使用定量实时聚合酶链反应（RT-qPCR）检测HSC活化标志物α-平滑肌肌动蛋白（α-SMA）、I型胶原和纤连蛋白的表达水平。通过蛋白质印迹法检测α-SMA和I型胶原的蛋白质水平。

结果

TFL改善了感染（）大鼠的肝功能，表现为转氨酶、胆红素和胆汁酸水平降低。TFL还减轻了肝组织的病理变化。TFL还减少了大鼠肝脏中活化HSC的数量并下调了纤维化相关标志物（I/III型胶原、α-SMA）。在HSC-T6中，TFL通过降低α-SMA、I型胶原和纤连蛋白的mRNA水平抑制HSC活化，并降低α-SMA和I型胶原的蛋白质水平。