Optoretinography with actively stabilized adaptive optics optical coherence tomography.

Optoretinography (ORG) is the optical measurement of changes in the retina in response to light stimulation. Adaptive optics optical coherence tomography (AOOCT) records photoreceptor ORGs by measuring the physical changes in their outer segment lengths in response to light stimulation. The main difficulty in recording these nanometer-scale changes is constant eye motion. Typically, fast volume acquisitions are used with offline spatial registration to compensate for the effect of eye motion. Here, we present an alternate solution whereby an adaptive optics scanning light ophthalmoscope (AOSLO) is used to measure the eye motion and actively guide the AOOCT beam to compensate for eye motion in real time. This system's cellular-scale tracking offers unparalleled control over scanning raster size and shape, allowing for high-speed (up to 100 kHz) ORG acquisition from targeted locations. We validate the method by comparing cone classifications against those made with an established ORG approach.