Heaf D J, Kaijser L, Eklund B, Carlson L A
Eur J Clin Invest. 1977 Jun;7(3):195-9. doi: 10.1111/j.1365-2362.1977.tb01597.x.
Various doses of heparin were given as a single injection into the brachial artery of each of twelve fasting healthy males. Plasma lipolytic activity was measured in samples obtained before and at frequent time intervals after heparin injection, in the artery and the deep and superficial veins of the same forearm. As little as 0.15 U heparin produced a rapid and detectable release of lipolytic activity in both deep and superficial veins. A series of tenfold increases in the dose produced correspondingly greater releases in the veins but the increments in the release were smaller than the increments in the dose. Three repeated 15 U doses of heparin, separated by 30 min, in the same subject gave a high degree of reproducibility of the release of lipolytic activity in both deep and superficial veins. 90% of the lipase activity in the vein was inhibited by 1 M NaCl. In all subjects the release of lipolytic activity was higher in the deep vein, which predominantly drains muscle, than in the superficial vein, which drains mostly subcutaneous tissues such as skin and adipose tissue. This indicates that muscle is a tissue of considerable importance as a source of post heparin plasma lipolytic activity.
对12名空腹健康男性，每人经肱动脉单次注射不同剂量的肝素。在肝素注射前及注射后不同时间间隔，采集同一前臂动脉、深静脉和浅静脉的血样，测定血浆脂解活性。低至0.15单位的肝素就能使深静脉和浅静脉中的脂解活性迅速且可检测地释放。剂量呈10倍递增时，静脉中的释放量相应增加，但释放量的增加幅度小于剂量的增加幅度。在同一受试者中，每隔30分钟重复注射3次15单位的肝素，深静脉和浅静脉中脂解活性的释放具有高度可重复性。静脉中90%的脂肪酶活性被1摩尔/升的氯化钠抑制。在所有受试者中，主要引流肌肉的深静脉中的脂解活性释放高于主要引流皮肤和脂肪组织等皮下组织的浅静脉。这表明肌肉作为肝素后血浆脂解活性的来源是一个相当重要的组织。
