谨防宿主对噬菌体的免疫反应可能影响噬菌体疗法。

Beware of host immune responses towards bacteriophages potentially impacting phage therapy.

作者信息

Démoulins Thomas, Cherbuin Jérémy D R, Yimthin Thatcha, Eggerschwiler Lukas, Adnan Fazal, Jores Joerg

机构信息

Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.

出版信息

Vet Res. 2025 Aug 15;56(1):170. doi: 10.1186/s13567-025-01600-1.

DOI:10.1186/s13567-025-01600-1

PMID:40817079

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12357382/

Abstract

The rising spread of antimicrobial resistance challenges animal welfare, productivity and food supply with respect to bacterial infections in different livestock species. Bacteriophages offer alternative means to control infections with resistant bacteria. This work investigated the host immune responses towards lytic bacteriophages K and T1, acting respectively on Gram-positive or Gram-negative bacteria. We employed an ex vivo platform to decipher various immune responses elicited by primary blood cells of eight outbred cattle. Bacteriophage K was not recognized whereas bacteriophage T1 induced a profound immune response. Therefore, prior characterization of host immune responses towards therapeutic phages should be considered before therapy starts.

摘要

抗菌药物耐药性的不断传播对不同家畜物种的细菌感染的动物福利、生产力和食物供应构成挑战。噬菌体提供了控制耐药细菌感染的替代手段。这项工作研究了宿主对分别作用于革兰氏阳性菌或革兰氏阴性菌的裂解性噬菌体K和T1的免疫反应。我们采用了一个体外平台来解读八只远交牛的原代血细胞引发的各种免疫反应。噬菌体K未被识别，而噬菌体T1诱导了强烈的免疫反应。因此，在开始治疗前应考虑对宿主针对治疗性噬菌体的免疫反应进行预先表征。