Namba-Koide Naoko, Yoshida Yasuhiro, Nagaoka Noriyuki, Okihara Takumi, Kawata Yusuke, Ito Masahiro, Ito Takashi, Takeuchi-Hatanaka Kazu, Shinoda-Ito Yuki, Omori Kazuhiro, Yamamoto Tadashi, Takashiba Shogo
Department of Periodontics and Endodontics, Division of Dentistry, Okayama University Hospital, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
Department of Biomaterials and Bioengineering, Faculty of Dental Medicine, Hokkaido University, Sapporo, 060-8586, Japan.
BMC Oral Health. 2025 Aug 16;25(1):1333. doi: 10.1186/s12903-025-06666-z.
Preventing oral infections, such as oral caries and periodontal disease, helps reduce the risks of various systemic diseases. In this study, the polysaccharide pullulan produced by the black yeast Aureobasidium pullulans was modified in combination with the cationic surfactant cetylpyridinium chloride (CPC) to create a local drug delivery system, and its antibacterial potential on oral bacteria was examined in vitro.
Pullulan was phosphorylated at the CHOH residue of α6 in the maltotriose structure and mixed with CPC. Bacterial attachment of cariogenic Streptococcus mutans on hydroxyapatite plates (HAPs) treated with the phosphorylated pullulan (PP) and CPC compound (0.01% PP and 0.001- 0.03% CPC, and vice versa) was assessed by observing bacteria using a field emission scanning electron microscope (FE-SEM) and quantified through 16 S rRNA amplification via real-time polymerase chain reaction (PCR). Additionally, the quartz crystal microbalance (QCM) method was employed to evaluate the sustained release of CPC.
PP-CPC compound maintained significant bactericidal activity even at 0.01%, which is one-fifth of the conventional applicable concentration of CPC. Additionally, a residual mixture was detected by the hydroxyapatite sensor of the crystal oscillator microbalance detector, suggesting an unknown molecular interaction that enables the sustained release of CPC after attachment to hydroxyapatite.
The combination of PP and CPC may contribute to the low concentration and effective prevention of oral infections, such as dental caries.
预防口腔感染，如龋齿和牙周病，有助于降低各种全身性疾病的风险。在本研究中，对黑酵母出芽短梗霉产生的多糖普鲁兰多糖与阳离子表面活性剂氯化十六烷基吡啶（CPC）进行联合修饰，以创建局部给药系统，并在体外检测其对口腔细菌的抗菌潜力。
在麦芽三糖结构的α6的CHOH残基处对普鲁兰多糖进行磷酸化，并与CPC混合。通过场发射扫描电子显微镜（FE-SEM）观察细菌，评估变形链球菌在经磷酸化普鲁兰多糖（PP）和CPC化合物（0.01%PP和0.001 - 0.03%CPC，反之亦然）处理的羟基磷灰石板（HAPs）上的细菌附着情况，并通过实时聚合酶链反应（PCR）对16S rRNA扩增进行定量。此外，采用石英晶体微天平（QCM）方法评估CPC的缓释情况。
PP-CPC化合物即使在0.01%时也保持显著的杀菌活性，这是CPC传统适用浓度的五分之一。此外，晶体振荡器微天平检测器的羟基磷灰石传感器检测到残留混合物，表明存在未知的分子相互作用，使得CPC在附着于羟基磷灰石后能够持续释放。
PP和CPC的组合可能有助于低浓度有效预防龋齿等口腔感染。
