脂肪干细胞原位移植到小鼠附睾白色脂肪组织的实验方案。

Protocol for in situ transplantation of adipose stem cells into mouse epididymal white adipose tissue.

作者信息

Liao Xiyan, Zeng Qin, Zhang Haowei, Xie Limin, Deng Tuo

机构信息

Division of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

Department of Clinical Nutrition, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.

出版信息

STAR Protoc. 2025 Aug 15;6(3):104035. doi: 10.1016/j.xpro.2025.104035.

DOI:10.1016/j.xpro.2025.104035

PMID:40819366

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12390934/

Abstract

Adipose stem cells (ASCs) play a crucial role in maintaining adipose tissue homeostasis, but studying them in situ is technically challenging. Here, we present a protocol for the transplantation of ASCs into mouse epididymal white adipose tissue (eWAT). We describe steps for isolating and purifying ASCs, labeling them with a cell tracker, administering anesthesia, making an incision, and performing spot injections of ASCs into the eWAT of mice. This protocol offers broad applications for in vivo functional research of ASCs. For complete details on the use and execution of this protocol, please refer to Liao et al..

摘要

脂肪干细胞（ASC）在维持脂肪组织稳态中起关键作用，但在原位研究它们在技术上具有挑战性。在此，我们展示了一种将ASC移植到小鼠附睾白色脂肪组织（eWAT）中的方案。我们描述了分离和纯化ASC、用细胞追踪剂标记它们、实施麻醉、切口以及将ASC点状注射到小鼠eWAT中的步骤。该方案为ASC的体内功能研究提供了广泛的应用。有关该方案使用和实施的完整详细信息，请参考廖等人的研究。