Baima Filho Francisco de As, Mendonça Priscylla G, Morgantetti Giuliano F, Silva-Sousa Alice C, Battaglion Leonardo R, Silva Ramon Syrc, Cartagenes Maria do S de S, Engel Edgard E
Ribeirão Preto Medical School, University of São Paulo (FMRP-USP) Ribeirão Preto, SP, Brazil.
Federal University of Maranhão (UFMA) Maranhão, Brazil.
Am J Transl Res. 2025 Jul 25;17(7):5641-5653. doi: 10.62347/GZLN4148. eCollection 2025.
This study investigated the role of marginal sclerosis in the recovery of mechanical strength following curettage and grafting of osseous lesions. To date, it remains unclear as to whether marginal sclerosis fully or partially restores the mechanical resistance of the affected bone to compressive, flexural, and torsional loads.
Adult male rats (WISTAR albino strain) aged approximately 90-days were used. Bone defects were created in the tibiae, and the animals were subsequently euthanized at different time points for outcome analysis. Various assessment methods were employed, including radiography, histology, immunohistochemistry, microcomputed tomography (micro-CT), mechanical bending and torsion tests.
This study revealed a progressive thickening of the osseous window borders before complete closure and a significant correlation between the intensity of marginal sclerosis that was radiographically observed, the rate of bone formation on histology, and the newly formed bone volume on micro-CT. Radiographs revealed thin marginal sclerosis at seven days post surgery, with a significant increase in sclerosis thickness being observed at ten days, whereas micro-CT revealed proportions of new bone formation of 44.80% at seven days and 105.70% at ten days. Additionally, at seven days, the mechanical resistance of the operated bones to torsion and bending was comparable to that of the non-operated bones, thereby indicating a complete recovery of mechanical strength.
This study revealed that the appearance of marginal sclerosis around bone defects (as identified by radiography) is associated with the complete recovery of mechanical strength in rat tibiae under both bending and torsion.
本研究调查了边缘性骨硬化在骨病变刮除及植骨后机械强度恢复中的作用。迄今为止，边缘性骨硬化是完全还是部分恢复受影响骨骼对压缩、弯曲和扭转负荷的机械抵抗力仍不清楚。
使用约90日龄的成年雄性大鼠（WISTAR白化品系）。在胫骨上制造骨缺损，随后在不同时间点对动物实施安乐死以进行结果分析。采用了多种评估方法，包括放射照相、组织学、免疫组织化学、微型计算机断层扫描（micro-CT）、机械弯曲和扭转试验。
本研究显示，在骨窗完全闭合之前，其边缘逐渐增厚，并且放射照相观察到的边缘性骨硬化强度、组织学上的骨形成速率与micro-CT上的新形成骨体积之间存在显著相关性。放射照片显示术后7天边缘性骨硬化较薄，10天时硬化厚度显著增加，而micro-CT显示7天时新骨形成比例为44.80%，10天时为105.70%。此外，在7天时，手术骨对扭转和弯曲的机械抵抗力与未手术骨相当，从而表明机械强度完全恢复。
本研究表明，骨缺损周围边缘性骨硬化的出现（通过放射照相确定）与大鼠胫骨在弯曲和扭转情况下机械强度的完全恢复相关。
