Zhou Jing, Tian Yao, Yang Min, Hao Ting, Ma Jun, Wang Shengyu
Department of Intensive Care Unit, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, China.
Shaanxi Lifegen Co., Ltd, Xi'an, Shaanxi, China.
PeerJ. 2025 Aug 12;13:e19849. doi: 10.7717/peerj.19849. eCollection 2025.
The capacity of metagenomic sequencing-based diagnostics to fully identify infections have made them useful instruments in clinical practice. We introduce an interactive platform that runs on a local server-class hardware resource and implements a number of open-source programs.
RpNGS integrates an interactive tabular interface for the management of experimental processes, patient metadata, and automated sequencing analysis. This technology optimizes clinical reporting by autonomously generating standardized reports in Word format. We have utilized the platform on an artificial microbial community reference panel and several clinical metagenomics datasets from public databases to demonstrate the efficacy of this workflow.
RpNGS is an innovative, user-friendly standalone application designed to store laboratory data (including reagents, primers, contaminants and run configurations), manage clinical metadata, process FASTQ files and produce analytical and comparative reports (including Word documents) that can be readily reviewed and certified. Its interactive interface necessitates no programming expertise, rendering it an invaluable instrument for clinical metagenomic pathogen identification.
基于宏基因组测序的诊断方法能够全面识别感染，使其成为临床实践中的有用工具。我们引入了一个运行在本地服务器级硬件资源上并实现多个开源程序的交互式平台。
RpNGS集成了一个交互式表格界面，用于管理实验过程、患者元数据和自动化测序分析。该技术通过自动生成Word格式的标准化报告来优化临床报告。我们已将该平台应用于人工微生物群落参考面板以及来自公共数据库的多个临床宏基因组数据集，以证明此工作流程的有效性。
RpNGS是一款创新的、用户友好的独立应用程序，旨在存储实验室数据（包括试剂、引物、污染物和运行配置）、管理临床元数据、处理FASTQ文件并生成可随时审核和认证的分析报告及比较报告（包括Word文档）。其交互式界面无需编程专业知识，使其成为临床宏基因组病原体鉴定的宝贵工具。
