Kim Dongseok, Park Seo-Young, Lee Yi Qing, Kwon Yiyoung, Choe Yon Ho, Kim Mi Jin, Lee Dong-Yup
School of Chemical Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
iScience. 2025 Jul 18;28(8):113160. doi: 10.1016/j.isci.2025.113160. eCollection 2025 Aug 15.
Pediatric-onset Crohn's disease (pCD) is characterized by frequent relapses and long-term impacts on growth, requiring targeted strategies for disease monitoring and treatment. To characterize gut microbiome and mycobiome changes in pCD, we profiled bacterial and fungal communities across multiple gastrointestinal (stool, colon, and terminal ileum) in 30 pre- and post-treatment patients and 36 healthy controls. Microbial shifts in mucosal biopsies lagged behind those in stool, indicating site-specific responsiveness to treatment. Notably, erythrocyte sedimentation rate (ESR) and the simple endoscopic score for CD (SES-CD) were strongly associated with bacterial composition, while platelet count (PLT) and body mass index (BMI) correlated with fungal compositional changes. Twelve key genera were linked to clinical indices across different sample types, with emerging as a relapse-associated biomarker. These findings highlight the importance of spatial sampling in pCD and delineate microbiota-associated genera and clinical indicators that may guide future precision diagnostics and therapeutic monitoring strategies.
儿童期发病的克罗恩病（pCD）的特点是频繁复发以及对生长产生长期影响，这需要针对性的疾病监测和治疗策略。为了明确pCD中肠道微生物组和真菌组的变化，我们对30例治疗前和治疗后的患者以及36名健康对照者的多个胃肠道部位（粪便、结肠和回肠末端）的细菌和真菌群落进行了分析。黏膜活检中的微生物变化滞后于粪便中的变化，表明对治疗存在部位特异性反应。值得注意的是，红细胞沉降率（ESR）和克罗恩病简易内镜评分（SES-CD）与细菌组成密切相关，而血小板计数（PLT）和体重指数（BMI）与真菌组成变化相关。12个关键菌属与不同样本类型的临床指标相关，其中 作为复发相关生物标志物出现。这些发现凸显了在pCD中进行空间采样的重要性，并确定了可能指导未来精准诊断和治疗监测策略的微生物相关菌属和临床指标。
