黑腹果蝇乙醇脱氢酶（Adh）区域的遗传与细胞遗传学分析。
Genetic and cytogenetic analysis of the Adh region in Drosophila melanogaster.
作者信息
O'Donnell J, Mandel H C, Krauss M, Sofer W
出版信息
Genetics. 1977 Jul;86(3):553-66. doi: 10.1093/genetics/86.3.553.
Abstract
Eighteen Adh-negative mutations were selected with 1-pentyn-3-ol after feeding of formaldehyde. Twelve of the 18 were shown by cytological and genetic analysis to be deletions. Cytological examination of the deletions allowed us to localize the Adh gene to a region including bands 35B3-5 on the left arm of chromosome 2. The deletions were also used to order known visible loci located near Adh.--The vital loci near Adh were also investigated. A total of 109 lethal mutations were generated with EMS and 33 of these, localized within a region defined by the overlap of two of the deletions, were found to belong to 13 complementation groups. If one includes three other loci known to belong there (el, Adh and Sco) a total of 16 complemetation groups have been identified in the region close to Adh.
摘要
在用甲醛喂养后，用1-戊炔-3-醇筛选出18个Adh阴性突变体。经细胞学和遗传学分析，这18个突变体中有12个为缺失突变。对这些缺失突变体进行细胞学检查，使我们能够将Adh基因定位到2号染色体左臂上包括35B3-5带的区域。这些缺失突变还被用于对位于Adh附近的已知可见基因座进行排序。——还对Adh附近的重要基因座进行了研究。用EMS产生了总共109个致死突变，其中33个定位在由两个缺失突变的重叠区域所定义的区域内，发现它们属于13个互补群。如果把另外三个已知属于该区域的基因座（el、Adh和Sco）包括在内，在Adh附近的区域总共鉴定出了16个互补群。
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