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一种利用雄性连锁易位进行等位基因系列分离的简单方法。

A simple method for the isolation of allelic series using male-linked translocations.

机构信息

Insect Genetics Unit, Research Institute ITAL, P.O. Box 48, NL-6700, AA Wageningen, The Netherlands.

出版信息

Theor Appl Genet. 1984 Feb;67(4):305-6. doi: 10.1007/BF00272865.

DOI:10.1007/BF00272865
PMID:24258650
Abstract

Using Adh null alleles a genetic sexing technique is being developed in Ceratitis capitata. In order to facilitate the isolation of a whole series of null alleles at this locus a technique utilizing male-linked translocations is described. It provides a simple efficient and general method for the isolation of any allelic series in species where little genetic information is available.

摘要

利用 Adh 无效等位基因，正在开发一种在黑腹果蝇中的遗传性别鉴定技术。为了方便在此基因座上分离一系列完整的无效等位基因，描述了一种利用雄性连锁易位的技术。它为在遗传信息很少的物种中分离任何等位基因系列提供了一种简单、高效和通用的方法。

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引用本文的文献

1
Genetic sexing in the mediterranean fruit fly, Ceratitis capitata, using the alcohol dehydrogenase locus.利用醇脱氢酶基因座对地中海实蝇进行遗传性别鉴定。
Theor Appl Genet. 1986 Jul;72(4):455-7. doi: 10.1007/BF00289526.
2
Induction of alcohol dehydrogenase null mutants in the Mediterranean fruit fly Ceratitis capitata.地中海实蝇（Ceratitis capitata）中乙醇脱氢酶缺失突变体的诱导
Biochem Genet. 1986 Oct;24(9-10):765-74. doi: 10.1007/BF00499008.

本文引用的文献

1
Genetic sexing in Drosophila melanogaster using the alcohol dehydrogenase locus and a Y-linked translocation.利用酒精脱氢酶基因座和 Y 连锁易位进行黑腹果蝇的遗传性别鉴定。
Theor Appl Genet. 1981 Jan;59(1):23-4. doi: 10.1007/BF00275770.
2
The genetics of a small autosomal region of Drosophila melanogaster, including the structural gene for alcohol dehydrogenase. V. Characterization of X-ray-induced Adh null mutations.黑腹果蝇一个常染色体小区域的遗传学，包括乙醇脱氢酶的结构基因。V. X射线诱导的Adh无效突变的特征分析
Genetics. 1982 Nov;102(3):421-35. doi: 10.1093/genetics/102.3.421.
3
Sex-ratio manipulation in relation to insect pest control.
与害虫防治相关的性别比例操纵
Annu Rev Genet. 1983;17:191-214. doi: 10.1146/annurev.ge.17.120183.001203.
4
Chemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-pentyne-3-ol.果蝇中影响乙醇脱氢酶的突变体的化学筛选。II. 1-戊炔-3-醇的应用。
Genetics. 1975 Jan;79(1):73-83. doi: 10.1093/genetics/79.1.73.
5
Chemical selection of mutants that affect ADH activity in Drosophila. III. Effects of ethanol.果蝇中影响乙醇脱氢酶活性的突变体的化学筛选。III. 乙醇的作用。
Biochem Genet. 1976 Feb;14(1-2):127-35. doi: 10.1007/BF00484879.
6
Genetic and cytogenetic analysis of the Adh region in Drosophila melanogaster.黑腹果蝇乙醇脱氢酶（Adh）区域的遗传与细胞遗传学分析。
Genetics. 1977 Jul;86(3):553-66. doi: 10.1093/genetics/86.3.553.
7
X-ray-induced mutations affecting the level of the enzyme alcohol dehydrogenase in Drosophila melanogaster: frequency and genetic analysis of null-enzyme mutants.X射线诱导影响黑腹果蝇乙醇脱氢酶水平的突变：无酶突变体的频率及遗传分析
Mutat Res. 1979 Nov;63(1):127-37. doi: 10.1016/0027-5107(79)90109-x.
8
The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. I. Characterization of deficiencies and mapping of ADH and visible mutations.黑腹果蝇一个包含乙醇脱氢酶结构基因的常染色体小区域的遗传学。I. 缺失的特征描述以及乙醇脱氢酶和可见突变的定位
Genetics. 1979 May;92(1):117-32. doi: 10.1093/genetics/92.1.117.