Fang Xufeng, Ji Ziqiao, Tao Min, Wang Xuezheng, Zhang Xian, Dai Zuyun, Yang Zhongzhou, Wang Chaonan, Zhu Zicheng, Liu Shi, Luan Feishi
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.
Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.
Theor Appl Genet. 2025 Aug 18;138(9):215. doi: 10.1007/s00122-025-05014-7.
The Cmwr locus was fine mapped to chromosome 2 in wrinkled-rind melon, and MELO3C010304.2 (CmNaa35, encoding an N-α-acetyltransferase) was identified as the most likely candidate gene for wrinkled rind in melon. The wrinkled rind (wr) phenotypic trait in melon is rare, and the regulatory mechanisms and genes governing this feature remain unclear. In this study, genetic analysis of a segregating F population derived from the wrinkled rind melon line T16-085 and the smooth rind line T15-022 suggested that the wr trait is controlled by a single recessive gene, designated Cmwr. Scanning electron microscopy suggested that rind wrinkling is caused by the uneven aggregation of rind cells. Bulked segregant analysis (BSA) delimited the candidate region to approximately 1.90 Mb on chromosome 2, genetic linkage analysis refined this locus to a 448.24-kb region, and fine mapping of data from 1140 F individuals restricted it to a 72.24-kb region containing eight candidate genes. The analysis of sequence variation in coding regions from the parental lines and eight melon germplasms with distinct rind morphologies indicated that MELO3C010304.2 (annotated as an N-α-acetyltransferase, CmNaa35) was the best candidate gene for the Cmwr locus. Two single nucleotide polymorphism (SNP) loci in the coding region (SNP and SNP) co-segregated with the wr trait. The analysis of promoter cis-acting elements and activity indicated that external indole-3-acetic acid treatment induced CmNaa35 expression. Our findings suggest that Cmwr is responsible for the wr trait in melon and provide genetic resources for further investigation, including gene function analysis and the exploration of the molecular mechanisms underlying melon rind wrinkling.
Cmwr基因座在皱皮甜瓜中被精细定位到2号染色体上，并且MELO3C010304.2（CmNaa35，编码一种N-α-乙酰基转移酶）被确定为甜瓜皱皮最可能的候选基因。甜瓜的皱皮（wr）表型性状较为罕见，控制该性状的调控机制和基因仍不清楚。在本研究中，对来自皱皮甜瓜品系T16-085和平滑皮品系T15-022的分离F群体进行遗传分析，结果表明wr性状由一个单隐性基因控制，命名为Cmwr。扫描电子显微镜显示，果皮起皱是由果皮细胞不均匀聚集引起的。混合分组分析法（BSA）将候选区域限定在2号染色体上约1.90 Mb的范围内，遗传连锁分析将该基因座精细定位到一个448.24 kb的区域，对1140个F个体的数据进行精细定位后将其限制在一个包含8个候选基因的72.24 kb区域。对亲本系和8种具有不同果皮形态的甜瓜种质编码区序列变异的分析表明，MELO3C010304.2（注释为N-α-乙酰基转移酶，CmNaa35）是Cmwr基因座的最佳候选基因。编码区的两个单核苷酸多态性（SNP）位点（SNP和SNP）与wr性状共分离。对启动子顺式作用元件和活性的分析表明，外源吲哚-3-乙酸处理可诱导CmNaa35表达。我们的研究结果表明，Cmwr基因导致了甜瓜的wr性状，并为进一步研究提供了遗传资源，包括基因功能分析和甜瓜果皮起皱分子机制的探索。
