Kemp M, Husby S, Jensenius J C, Rasmussen G G, Svehag S E
Acta Pathol Microbiol Immunol Scand C. 1985 Oct;93(5):217-23. doi: 10.1111/j.1699-0463.1985.tb02948.x.
A one day enzyme immunoassay for the detection of rheumatoid factors of the IgG, IgM, and IgA class is described. The assay utilizes rabbit IgG as solid-phase reactant and the biotin-avidin interaction for the coupling of enzyme to indicator antibody. Three different indicator antibodies discriminated effectively between rheumatoid arthritis patients and normal subjects. F(ab')2 fragments of goat antibodies were found best suited for the test. Rheumatoid factor activity was expressed in U/ml by comparing samples to an internal standard, which was related to the WHO international reference serum for rheumatoid arthritis. Rheumatoid factor activity (U/ml) in the IgM-specific assay showed a close correlation to the latex agglutination titer. Avidity indices estimated from the slopes of the dose response curves of test sera were significantly higher for rheumatoid arthritis patients than for a group of healthy persons, indicating a higher avidity of the rheumatoid factors in the patients' sera.
本文描述了一种用于检测IgG、IgM和IgA类类风湿因子的一日酶免疫测定法。该测定法利用兔IgG作为固相反应物，并利用生物素-抗生物素蛋白相互作用将酶与指示抗体偶联。三种不同的指示抗体有效地区分了类风湿性关节炎患者和正常受试者。发现山羊抗体的F(ab')2片段最适合该测试。通过将样品与内标进行比较，类风湿因子活性以U/ml表示，该内标与世界卫生组织类风湿性关节炎国际参考血清相关。IgM特异性测定中的类风湿因子活性（U/ml）与乳胶凝集滴度密切相关。从测试血清的剂量反应曲线斜率估计的亲和力指数在类风湿性关节炎患者中明显高于一组健康人，表明患者血清中类风湿因子的亲和力更高。
