Asbakk K, Iversen O J
Department of Microbiology, University of Trondheim, Norway.
Acta Pathol Microbiol Immunol Scand C. 1987 Aug;95(4):161-6. doi: 10.1111/j.1699-0463.1987.tb00025.x.
An isotype-specific ELISA for detection of rheumatoid factors (RFs) is described. The assay utilizes rabbit anti-albumin IgG bound to albumin as solid phase reactant (test wells). RF-activity was expressed as the difference in reaction between test wells and wells containing F(ab')2-fragments of the rabbit IgG bound to albumin. The activity correlated well with the conventional Waaler agglutination test on sera that showed a positive Waaler titre, but several sera that were negative by the Waaler test were positive for RF of IgG isotype by the ELISA-test. The method was applied on specimens from psoriatic patients, and RFs of IgG- and IgA isotype were demonstrated in serum immune complexes from all the psoriatic patients examined. IgG- and IgA-RF was also demonstrated in extracts from psoriatic scale, and RFs in scale were demonstrated independently of free rheumatoid factor activity in serum.
本文描述了一种用于检测类风湿因子（RF）的同型特异性酶联免疫吸附测定法（ELISA）。该检测方法利用与白蛋白结合的兔抗白蛋白IgG作为固相反应物（检测孔）。RF活性通过检测孔与含有与白蛋白结合的兔IgG的F(ab')2片段的孔之间的反应差异来表示。对于显示阳性瓦勒尔滴度的血清，该活性与传统的瓦勒尔凝集试验相关性良好，但通过ELISA检测，一些瓦勒尔试验呈阴性的血清对IgG同型的RF呈阳性。该方法应用于银屑病患者的标本，在所检测的所有银屑病患者的血清免疫复合物中均检测到IgG和IgA同型的RF。在银屑病鳞屑提取物中也检测到IgG和IgA-RF，并且鳞屑中的RF与血清中游离类风湿因子活性无关。
