Karimi Fatemeh, Kafami Marzieh, Khakestar Fatemeh Ghodrati, Madahali Mohammad Hossein, Nazemi Samad, Karimi Sareh
Student Research Committee, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Department of Physiology and Pharmacology, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Mol Cell Biochem. 2025 Aug 19. doi: 10.1007/s11010-025-05371-3.
Fertility preservation is a critical aspect of improving human life quality. Sperm cryopreservation is highly recommended for men at risk of infertility; however, it can have detrimental effects on sperm quality. In this study, we evaluate the effects of platelet-rich plasma (PRP) and PRP-derived exosomes on cryopreservation outcomes in men with asthenoteratozoospermia referred to a public infertility center. The semen samples from 75 men with asthenoteratozoospermia (based on WHO 2020 criteria) were randomly divided into five groups: Control (conventional freezing), Exo/before, Exo/After, PRP/Before, and PRP/After. In all intervention groups, PRP or exosomes were added either 1 h before freezing or 1 h after thawing. Sperm parameters and oxidative stress were evaluated in all samples. Sperm incubation with exosomes before freezing significantly improves motility and lowers DNA fragmentation index (DFI) and oxidative stress compared to the control and other groups. Based on the present study, semen incubation with exosomes for 1 h before freezing not only protects sperm against oxidative stress but also enhances some sperm parameters, such as motility, in men with asthenoteratozoospermia.
生育力保存是提高人类生活质量的一个关键方面。对于有不孕风险的男性，强烈建议进行精子冷冻保存；然而，这可能会对精子质量产生不利影响。在本研究中，我们评估了富血小板血浆（PRP）和PRP衍生的外泌体对一家公立不孕不育中心收治的弱畸精子症男性冷冻保存结果的影响。将75名弱畸精子症男性（根据世界卫生组织2020年标准）的精液样本随机分为五组：对照组（传统冷冻）、外泌体/冷冻前、外泌体/解冻后、PRP/冷冻前和PRP/解冻后。在所有干预组中，在冷冻前1小时或解冻后1小时添加PRP或外泌体。对所有样本评估精子参数和氧化应激。与对照组和其他组相比，冷冻前将精子与外泌体孵育可显著提高精子活力，降低DNA碎片指数（DFI）和氧化应激。基于本研究，冷冻前将精液与外泌体孵育1小时不仅能保护精子免受氧化应激，还能改善弱畸精子症男性的一些精子参数（如活力）。
