Batool Sidra, Rafiq Khadija, Saeed Muhammad, Naeem Atyia, Chohan Muhammad Shahid Munir, Akram Muhammad Sohail
Department of Botany, Faculty of Life Sciences, Government College University, Faisalabad, 38000, Pakistan.
Department of Botany, University of Gujrat, Gujrat, Pakistan.
BMC Plant Biol. 2025 Aug 19;25(1):1095. doi: 10.1186/s12870-025-07183-5.
Cotton is considered moderately salt-tolerant, yet the degree of tolerance can vary significantly among different genotypes.
The current study examined the effects of plant growth-promoting bacterium "Achromobacter sp. FB-14" in two cotton genotypes (FH-326 and FH-941). Under 150 mM salt stress, FH-941 showed a higher accumulation of malondialdehyde (MDA) and hydrogen peroxide (HO) i.e. 24.4 and 8.20 µmol g FW, respectively. Contrarily, FH-326 exhibited higher catalase (CAT) and peroxidase (POD) activities (48.82 & 27.81 Umg protein, respectively). FB-14 inoculated plants exhibited an increase in chlorophyll a content by 10% (FH-326) and 8% (FH-941). FH-326 exhibited better ion (Na, K, Ca, Mg) uptake and root-shoot translocation patterns than FH-941. Imposition of salt stress, without FB-14 inoculation, resulted in higher reduction in NHX1, SOS1, and HAK5 gene expression in FH-941 compared to FH-326. While elevated expression of HKT1 was recorded in FH-326 compared to FH-941. A down-regulation of LHCB, GhWRKY3 and GhWRKY34 was recorded in both genotypes except GhWRKY34 was up-regulated in FH-326. Additionally, FB-14 inoculation in FH-941, under salt stress, led to up-regulation of LHCB and GhWRKY3 and GhWRKY34.
The FH-326 maintained optimum HO and MDA content through POD/CAT activities, balanced ion uptake and gene regulation. The growth of salt-sensitive FH-941 was facilitated by FB-14 inoculation through modulated patterns of ion homeostasis, expression of ion transporter and transcription factors. Further studies involving more cotton genotypes and salt-related molecular markers under bacterial inoculation may assist in devising better strategies to achieve potential yield of cotton.
棉花被认为具有中等耐盐性，但不同基因型之间的耐受程度可能有显著差异。
本研究考察了植物促生细菌“无色杆菌属FB - 14”对两种棉花基因型（FH - 326和FH - 941）的影响。在150 mM盐胁迫下，FH - 941的丙二醛（MDA）和过氧化氢（HO）积累量较高，分别为24.4和8.20 μmol g鲜重。相反，FH - 326表现出较高的过氧化氢酶（CAT）和过氧化物酶（POD）活性（分别为48.82和27.81 Umg蛋白）。接种FB - 14的植株叶绿素a含量分别增加了10%（FH - 326）和8%（FH - 941）。FH - 326比FH - 941表现出更好的离子（钠、钾、钙、镁）吸收和根茎转运模式。在未接种FB - 14的情况下施加盐胁迫，与FH - 326相比，FH - 941中NHX1、SOS1和HAK5基因表达的降低幅度更大。与FH - 941相比，FH - 326中HKT1的表达升高。除了FH - 326中GhWRKY34上调外，两种基因型中LHCB、GhWRKY3和GhWRKY34均下调。此外，在盐胁迫下，对FH - 941接种FB - 14导致LHCB、GhWRKY3和GhWRKY34上调。
FH - 326通过POD/CAT活性、平衡的离子吸收和基因调控维持了最佳的HO和MDA含量。通过调节离子稳态模式、离子转运体和转录因子的表达，接种FB - 14促进了盐敏感型FH - 941的生长。进一步涉及更多棉花基因型和细菌接种下与盐相关分子标记的研究，可能有助于制定更好的策略以实现棉花的潜在产量。
