Raff M J, Summersgill J T, Fontana F J, Barnwell P A, Waterman N G, Scharfenberger L
J Antibiot (Tokyo). 1977 Jul;30(7):593-6. doi: 10.7164/antibiotics.30.593.
Minocycline was added to normal and hyperlipemic serum samples in concentrations of 1 approximately 10 mcg/ml. These specimens had similar protein contents. Chemically extractable minocycline was quantitated fluorometrically. Hyperlipemic serum (cholesterol 480 mg/100 ml; triglycerides 321 mg/100 ml) yielded an average of 50% less minocycline than did normal serum (cholesterol 170 mg/100 ml; triglycerides 114 mg/100 ml). When ultrafiltrates of serum containing 6, 12 and 20 mcg/ml minocycline were assayed microbiologically, it was evident that variations in serum triglyceride and cholesterol levels did not alter the ratio of bound to free drug. Minocycline appears to be reversibly associated with, and/or soluble in, triglyceride-cholesterol components of serum.
将米诺环素以约1至10微克/毫升的浓度添加到正常血清样本和高脂血症血清样本中。这些样本的蛋白质含量相似。采用荧光法对化学可提取的米诺环素进行定量分析。高脂血症血清（胆固醇480毫克/100毫升；甘油三酯321毫克/100毫升）产生的米诺环素平均比正常血清（胆固醇170毫克/100毫升；甘油三酯114毫克/100毫升）少50%。当对含有6、12和20微克/毫升米诺环素的血清超滤物进行微生物学检测时，很明显血清甘油三酯和胆固醇水平的变化不会改变结合型药物与游离型药物的比例。米诺环素似乎与血清中的甘油三酯 - 胆固醇成分可逆性结合和/或可溶于其中。
