De Leenheer A P, Nelis H J
J Pharm Sci. 1979 Dec;68(12):1527-30. doi: 10.1002/jps.2600681217.
A rapid, specific, and sensitive liquid chromatographic assay for minocycline in human serum is described. The drug and an internal standard (oxytetracycline) were extracted into ethyl acetate from 0.5 ml of buffered serum (pH 6.5). Chromatographic separation was achieved on a 10-micrometer Lichrospher 100 CH 8 column with acetonitrile--citric acid (0.1 M) as the eluent. The column effluent was monitored at 352 nm. The assay was linear up to 3 micrograms/ml with a mean coefficient of variation of 3.3% (n = 6). An extraction recovery of 89.4 +/- 3.2% (mean +/- SD, n = 17) was obtained over the 0.5--2.6 micrograms/ml range. The detection limit averaged 50 ng/ml. A serum concentration-time profile in humans after oral intake is presented.
本文描述了一种用于测定人血清中米诺环素的快速、特异且灵敏的液相色谱法。将药物和内标（土霉素）从0.5 ml缓冲血清（pH 6.5）中萃取到乙酸乙酯中。在10微米的Lichrospher 100 CH 8柱上，以乙腈 - 柠檬酸（0.1 M）作为洗脱剂进行色谱分离。在352 nm处监测柱流出物。该测定法在高达3微克/毫升时呈线性，平均变异系数为3.3%（n = 6）。在0.5 - 2.6微克/毫升范围内，萃取回收率为89.4 +/- 3.2%（平均值 +/- 标准差，n = 17）。检测限平均为50纳克/毫升。给出了人体口服给药后的血清浓度 - 时间曲线。
