Dardalas Ioannis, Lagoudaki Roza, Kosmidis Efstratios K, Kimiskidis Vasilios, Moysiadis Theodoros, Kouvelas Dimitrios, Pourzitaki Chryssa
Laboratory of Clinical Pharmacology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, GRC.
Laboratory of Physiology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, GRC.
Cureus. 2025 Jul 19;17(7):e88284. doi: 10.7759/cureus.88284. eCollection 2025 Jul.
Introduction Valproic acid (VA) has been a widely used drug in the management of epilepsy, migraine headaches, and bipolar disorder for many decades. Recently, the drug has been tested in various types of malignancy, showing promising results. Despite its well-established effectiveness, there are certain safety concerns that should be taken into consideration. VA has been classified as teratogenic and should be avoided during pregnancy. Methods This study was designed in order to present the effects of valproate on differentiated SH-SY5Y cell cultures and their concentration dependency. SH-SY5Y cells were differentiated into a mature neuron-like phenotype and then treated with VA at either 1 mM or 10 mM. Morphological criteria were compared between treated and untreated cells, serving as a control for identification. Results It was observed that VA consistently induces extensive detachment of the adherent cells and cell death. At a concentration of 1 mM, a 44,0% reduction of adherent cells was observed, while at a concentration of 10 mM, the reduction was 95.9%. Conclusion VA causes extensive cell detachment and death in differentiated SH-SY5Y cell cultures. There is a paucity of studies on these effects, which underlines the importance of further and more extensive research on this matter.
引言 几十年来，丙戊酸（VA）一直是治疗癫痫、偏头痛和双相情感障碍的常用药物。最近，该药物在各类恶性肿瘤中进行了试验，显示出了有前景的结果。尽管其有效性已得到充分证实，但仍有一些安全问题需要考虑。VA已被归类为致畸剂，孕期应避免使用。
方法 本研究旨在呈现丙戊酸盐对分化的SH-SY5Y细胞培养物的影响及其浓度依赖性。将SH-SY5Y细胞分化为成熟的神经元样表型，然后用1 mM或10 mM的VA进行处理。比较处理组和未处理组细胞的形态学标准，未处理组作为对照用于鉴定。
结果 观察到VA持续诱导贴壁细胞广泛脱离和细胞死亡。在1 mM浓度下，观察到贴壁细胞减少了44.0%，而在10 mM浓度下，减少了95.9%。
结论 VA在分化的SH-SY5Y细胞培养物中导致广泛的细胞脱离和死亡。关于这些影响的研究较少，这凸显了对此问题进行进一步更广泛研究的重要性。
