Zhao Yefang, Wang Bingjie, Wei Xujing, Liu Dengxiang, Wang Ruiping, Ma Huashu, Qiao Zongxu, Kong Nana, Feng Jinhui, Cui Dan, Hou Shaoke, Zhang Hongzhen
Department of Obstetrics, Xingtai People's Hospital, Affiliated Hospital of Hebei Medical University, Xingtai, Hebei, China.
Department of Proctology, Xingtai People's Hospital, Affiliated Hospital of Hebei Medical University, Xingtai, Hebei, China.
Mediators Inflamm. 2025 Aug 11;2025:3010379. doi: 10.1155/mi/3010379. eCollection 2025.
This study aimed to investigate the impact of fecal microbiota transplantation (FMT) on gut microbiota composition and serum inflammatory factors in a murine model. Female C57BL/6J mice ( = 60) were divided into four groups: control (Con), negative (Neg), normal transplantation (NT), and preeclampsia transplantation (PET). The Con group received no treatment, while the Neg, NT, and PET groups were administered a triple antibiotic regimen (ampicillin, neomycin sulfate, and metronidazole) for 14 days to deplete gut microbiota. Following antibiotic treatment, FMT was performed: the NT group received fecal microbiota from healthy pregnant women and the PET group received microbiota from severe preeclampsia patients. Fecal samples and serum were collected for 16S rRNA sequencing and inflammatory factor analysis, respectively. Significant differences in gut microbial composition were observed between the PET group and other groups, with enriched taxa such as , , and in the PET group. Conversely, taxa such as and were more abundant in the fecal microbiota of mice in the NT group. Furthermore, serum levels of lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) were markedly elevated in the PET group compared to the control, negative, and NT groups. Transplantation with fecal bacteria from preeclampsia patients leads to significant alterations in gut microbiota composition and increased serum inflammatory factors levels in mice. These findings provide insights into the relationship between gut microbiota and inflammatory processes in preeclampsia and underscore the potential therapeutic implications of FMT in modulating gut microbiota dysbiosis and inflammatory responses.
本研究旨在探讨粪便微生物群移植（FMT）对小鼠模型肠道微生物群组成和血清炎症因子的影响。将60只雌性C57BL/6J小鼠分为四组：对照组（Con）、阴性组（Neg）、正常移植组（NT）和子痫前期移植组（PET）。Con组不接受任何治疗，而Neg、NT和PET组接受三联抗生素方案（氨苄青霉素、硫酸新霉素和甲硝唑）治疗14天以耗尽肠道微生物群。抗生素治疗后，进行FMT：NT组接受来自健康孕妇的粪便微生物群，PET组接受来自重度子痫前期患者的微生物群。分别收集粪便样本和血清进行16S rRNA测序和炎症因子分析。PET组与其他组之间观察到肠道微生物组成存在显著差异，PET组中 、 和 等分类群富集。相反，NT组小鼠粪便微生物群中 和 等分类群更为丰富。此外，与对照组、阴性组和NT组相比，PET组血清中脂多糖（LPS）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平明显升高。移植子痫前期患者的粪便细菌会导致小鼠肠道微生物群组成发生显著改变，并增加血清炎症因子水平。这些发现为子痫前期肠道微生物群与炎症过程之间的关系提供了见解，并强调了FMT在调节肠道微生物群失调和炎症反应方面的潜在治疗意义。
