Department of Obstetrics and Gynecology, First Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Department of Obstetrics, Xingtai People's Hospital, Affiliated Hospital of Hebei Medical University, Xingtai, Hebei, China.
Front Cell Infect Microbiol. 2023 Jan 4;12:1022857. doi: 10.3389/fcimb.2022.1022857. eCollection 2022.
To compare the difference of gut microbiota between preeclampsia (PE) and healthy normal pregnant women, providing new therapeutic strategy for preeclampsia.
Forty-one PE patients and 45 age- and pre-pregnancy body mass index- matched healthy controls were enrolled from Nov 2021 to May 2022 in this retrospective case-control study. Fecal microbiota was detected by 16S rRNA gene sequencing, followed by bioinformatics analysis including microbial α diversity, microbial β diversity, and linear discriminant analysis effect size (LEfSe) analysis. Serum inflammatory factors were also detected and compared between the two groups.
There were significant differences in Bacteroidetes (2.68% in PE patients vs 11.04% in healthy controls, 0.001), Proteobacteria (4.04% in PE patients vs 1.22% in healthy controls, = 0.041), and Fusobacteria (1.07% in PE patients vs 0.01% in healthy controls, = 0.042) between the two groups at the phylum level. Microbial α diversity was lower in PE patients than that in healthy controls. In addition, there was significant difference in microbial β diversity between the two groups. LEfSe analysis showed that there are 24 different taxa between the two groups. The levels of proinflammatory factors including serum tumor necrosis factor-α and Interleukin-6 were statistically significant higher in PE patients than those in healthy controls (both < 0.001), while there were no significant differences in the levels of serum anti-inflammatory factors including Interleukin-4 and Interleukin-10 between the two groups ( = 0.234 and = 0.096, respectively).
PE patients demonstrated gut microbiota disturbances and increasing serum proinflammatory factors, leading to a better understanding of the relationship between the gut microbiota dysbiosis and PE.
比较先兆子痫（PE）与健康正常孕妇之间肠道微生物群的差异，为先兆子痫提供新的治疗策略。
本回顾性病例对照研究于 2021 年 11 月至 2022 年 5 月纳入 41 例 PE 患者和 45 例年龄和孕前体重指数匹配的健康对照者。采用 16S rRNA 基因测序检测粪便微生物群，然后进行生物信息学分析，包括微生物 α多样性、微生物β多样性和线性判别分析效应量（LEfSe）分析。还检测了两组之间的血清炎症因子并进行了比较。
两组在门水平上有显著差异，厚壁菌门（PE 患者 2.68%，健康对照者 11.04%， = 0.001）、变形菌门（PE 患者 4.04%，健康对照者 1.22%， = 0.041）和梭杆菌门（PE 患者 1.07%，健康对照者 0.01%， = 0.042）。PE 患者的微生物 α多样性低于健康对照组。此外，两组之间的微生物β多样性存在显著差异。LEfSe 分析显示，两组之间有 24 个不同的分类群。PE 患者的促炎因子包括血清肿瘤坏死因子-α和白细胞介素-6的水平明显高于健康对照组（均 < 0.001），而两组之间血清抗炎因子包括白细胞介素-4 和白细胞介素-10 的水平无显著差异（分别为 = 0.234 和 = 0.096）。
PE 患者表现出肠道微生物群紊乱和血清促炎因子增加，这有助于更好地理解肠道微生物群失调与 PE 之间的关系。
