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使用磁激活细胞分选技术从单只小鼠大脑中分离少突胶质细胞、小胶质细胞、内皮细胞、星形胶质细胞和神经元的实验方案。

Protocol to isolate oligodendrocytes, microglia, endothelial cells, astrocytes, and neurons from a single mouse brain using magnetic-activated cell sorting.

作者信息

Houmam Samah, Siodlak Dominika, Pham Kevin D, Salinas-Salinas Casandra, Ocañas Sarah R, Freeman Willard M, Rice Heather C

出版信息

bioRxiv. 2025 Aug 12:2025.08.08.666877. doi: 10.1101/2025.08.08.666877.

DOI:10.1101/2025.08.08.666877
PMID:40832248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12363854/
Abstract

The isolation of specific cell types of the brain is essential to study cell-type-specific differences in complex neurological diseases such as Alzheimer's disease. This protocol isolates oligodendrocytes, microglia, endothelial cells, astrocytes, and neurons from a single mouse brain. The process involves gentle tissue homogenization, debris removal, and sequential sorting of five distinct cell types. We validate cell purity and viability using flow cytometry and RT-qPCR. This protocol is well-suited for a range of downstream applications, including genomics, transcriptomics, and proteomics.

摘要

分离大脑中的特定细胞类型对于研究诸如阿尔茨海默病等复杂神经疾病中的细胞类型特异性差异至关重要。本方案可从单个小鼠大脑中分离少突胶质细胞、小胶质细胞、内皮细胞、星形胶质细胞和神经元。该过程包括轻柔的组织匀浆、去除碎片以及对五种不同细胞类型进行顺序分选。我们使用流式细胞术和逆转录定量聚合酶链反应（RT-qPCR）来验证细胞纯度和活力。本方案非常适合一系列下游应用，包括基因组学、转录组学和蛋白质组学。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/d40b5b88f724/nihpp-2025.08.08.666877v1-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/16fb6acb7e2c/nihpp-2025.08.08.666877v1-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/261c75bc8060/nihpp-2025.08.08.666877v1-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/54776d085bd3/nihpp-2025.08.08.666877v1-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/858d99aad622/nihpp-2025.08.08.666877v1-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/d40b5b88f724/nihpp-2025.08.08.666877v1-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/16fb6acb7e2c/nihpp-2025.08.08.666877v1-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/261c75bc8060/nihpp-2025.08.08.666877v1-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/54776d085bd3/nihpp-2025.08.08.666877v1-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/858d99aad622/nihpp-2025.08.08.666877v1-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd20/12363854/d40b5b88f724/nihpp-2025.08.08.666877v1-f0006.jpg

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本文引用的文献

1
(Re)building the nervous system: A review of neuron-glia interactions from development to disease.（重新）构建神经系统：从发育到疾病的神经胶质细胞相互作用综述
J Neurochem. 2025 Jan;169(1):e16258. doi: 10.1111/jnc.16258.
2
The proteogenomic landscape of multiple myeloma reveals insights into disease biology and therapeutic opportunities.多发性骨髓瘤的蛋白质基因组全景揭示了疾病生物学和治疗机会的新见解。
Nat Cancer. 2024 Aug;5(8):1267-1284. doi: 10.1038/s43018-024-00784-3. Epub 2024 Jun 28.
3
Practical Considerations for Single-Cell Genomics.
单细胞基因组学的实际考虑因素。
Curr Protoc. 2022 Aug;2(8):e498. doi: 10.1002/cpz1.498.
4
Minimizing the Confounds of Cell-Isolation Techniques on Transcriptomic and Translatomic Profiles of Purified Microglia.最小化细胞分离技术对纯化小胶质细胞转录组和翻译组谱的干扰。
eNeuro. 2022 Mar 28;9(2). doi: 10.1523/ENEURO.0348-21.2022. Print 2022 Mar-Apr.
5
Single-cell transcriptome profiling reveals vascular endothelial cell heterogeneity in human skin.单细胞转录组分析揭示了人类皮肤中血管内皮细胞的异质性。
Theranostics. 2021 Apr 19;11(13):6461-6476. doi: 10.7150/thno.54917. eCollection 2021.
6
One Brain-All Cells: A Comprehensive Protocol to Isolate All Principal CNS-Resident Cell Types from Brain and Spinal Cord of Adult Healthy and EAE Mice.一脑-全细胞：从成年健康和 EAE 小鼠的大脑和脊髓中分离所有主要中枢神经系统驻留细胞类型的综合方案。
Cells. 2021 Mar 15;10(3):651. doi: 10.3390/cells10030651.
7
Microglial gene signature reveals loss of homeostatic microglia associated with neurodegeneration of Alzheimer's disease.小胶质细胞基因特征揭示了与阿尔茨海默病神经退行性变相关的稳态小胶质细胞的丧失。
Acta Neuropathol Commun. 2021 Jan 5;9(1):1. doi: 10.1186/s40478-020-01099-x.
8
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Curr Protoc Neurosci. 2019 Jun;88(1):e71. doi: 10.1002/cpns.71.
9
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Genome Biol. 2018 Aug 10;19(1):110. doi: 10.1186/s13059-018-1496-z.
10
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JCI Insight. 2018 Jul 12;3(13):121109. doi: 10.1172/jci.insight.121109.