Genomics of Gene Expression Laboratory, Centro de Investigación Principe Felipe (CIPF), 46012, Valencia, Spain.
Department of Microbiology and Cell Science, Institute for Food and Agricultural Sciences, Genetics Institute, University of Florida, Gainesville, Florida, 32611, USA.
Genome Biol. 2018 Aug 10;19(1):110. doi: 10.1186/s13059-018-1496-z.
Single-cell RNAseq and alternative splicing studies have recently become two of the most prominent applications of RNAseq. However, the combination of both is still challenging, and few research efforts have been dedicated to the intersection between them. Cell-level insight on isoform expression is required to fully understand the biology of alternative splicing, but it is still an open question to what extent isoform expression analysis at the single-cell level is actually feasible. Here, we establish a set of four conditions that are required for a successful single-cell-level isoform study and evaluate how these conditions are met by these technologies in published research.
单细胞 RNA 测序和可变剪接研究最近已成为 RNA 测序的两个最突出的应用之一。然而，两者的结合仍然具有挑战性，很少有研究致力于两者的交叉点。在单细胞水平上对异构体表达的细胞水平的洞察对于充分理解可变剪接的生物学是必需的，但在单细胞水平上进行异构体表达分析实际上可行的程度仍然是一个悬而未决的问题。在这里，我们建立了一套在单细胞水平上成功进行异构体研究所需的四个条件，并评估了这些技术在已发表的研究中满足这些条件的程度。
