Ding Ning, Xiang Ben, Jiang Huiwen, Yu Yao, Liu Jian, Zhao Yuqian, Luan Jingmin, Yang Yanqiu, Wang Yi, Ma Zhenhe
College of Information Science and Engineering, Northeastern University, Shenyang, China.
School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao, China.
J Cereb Blood Flow Metab. 2025 Aug 20:271678X251369613. doi: 10.1177/0271678X251369613.
Early diagnosis of ischemic stroke is crucial for timely intervention and saving brain function. The mechanisms of stroke are complex, involving multiple parameters such as blood flow perfusion, tissue status, and oxygenation levels. These parameters interact with each other, necessitating multiple systems to evaluate. The animal model must be moved among systems for various systems monitoring, which complicates the operation and may induce additional interference. Here, we propose a non-contact all-optic OCT-PAM dual-mode imaging system for monitoring rat cortex states. The compact probe enables facilitating long-term multi-parameter observation. Using the system, we quantitatively monitored the state of the rat cortex of ischemic stroke during the acute phase, including blood perfusion density, vessel diameter, vessel length, optical attenuation coefficient, and photoacoustic absorption. We observed reduced perfusion in the affected cortex, contralateral perfusion increases, and vasodilation, suggesting collateral circulation activation. Additionally, increased optical attenuation and photoacoustic absorption in the stroke area were linked to blood-brain barrier changes. This OCT-PAM system and its quantitative analysis method offer a promising tool for early ischemic stroke diagnosis and exploring underlying molecular and cellular mechanisms.
缺血性中风的早期诊断对于及时干预和挽救脑功能至关重要。中风的机制复杂，涉及多个参数，如血流灌注、组织状态和氧合水平。这些参数相互作用，需要多个系统进行评估。动物模型必须在不同系统之间移动以进行各种系统监测，这使得操作变得复杂，并可能产生额外干扰。在此，我们提出一种用于监测大鼠皮层状态的非接触式全光学OCT-PAM双模成像系统。紧凑的探头便于进行长期多参数观察。使用该系统，我们定量监测了急性缺血性中风大鼠皮层的状态，包括血流灌注密度、血管直径、血管长度、光学衰减系数和光声吸收。我们观察到受影响皮层的灌注减少，对侧灌注增加以及血管舒张，提示侧支循环激活。此外，中风区域光学衰减和光声吸收的增加与血脑屏障变化有关。这种OCT-PAM系统及其定量分析方法为早期缺血性中风诊断和探索潜在的分子和细胞机制提供了一个有前景的工具。
