Liu Huachun, Cheng Yanfang, Zhou Hongwei, Pan Xinhong, Peng Rongfei, Bai Zhijun, Li Juntao, Tan Lei
Institute of Public Health, Guangzhou Medical University & Guangzhou Center for Disease Control and Prevention, Guangzhou, 510440, China.
School of Public Health, Guangzhou Medical University, Guangzhou 510515, China.
Anal Methods. 2025 Aug 28;17(34):6800-6809. doi: 10.1039/d5ay01201e.
Arsenic is a widely studied toxic element that exists in various species with different oxidation states and forms in the environment and biological systems. The different physicochemical properties, environmental behaviors, and toxicities of these arsenic species make speciation analysis essential for environmental monitoring and human health risk assessment. In this study, we demonstrated the determination and monitoring of six arsenic species, including arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenocholine (AsC), and arsenobetaine (AsB) in seafood using high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). We optimized the chromatographic condition and sample preparation for the separation and extraction of arsenic species, and the method was validated with accuracy and reproducibility using certified reference materials and actual samples. Baseline separation of the arsenic species was achieved within 12 min, and oven heat extraction was selected for sample preparation. The method showed excellent linearity for arsenic species from 0.1 to 100 μg L, with recoveries ranging from 92% to 123%, intra-day precision of 0.9-5.0%, and inter-day precision of 1.1-19.4%. The method was further used to determine the arsenic species in 109 mollusks, 195 crustaceans, and 195 marine fish samples from Guangdong, China. Distinctive patterns of arsenic species distribution were observed among different seafood categories. AsB was consistently the predominant species across all samples, with a detection frequency higher than 97.9% and contributed to more than 90% of total arsenic, other arsenic species showed marked variations in their occurrence and concentrations.
砷是一种被广泛研究的有毒元素，在环境和生物系统中以具有不同氧化态和形式的各种物种存在。这些砷物种不同的物理化学性质、环境行为和毒性使得形态分析对于环境监测和人类健康风险评估至关重要。在本研究中，我们展示了使用高效液相色谱-电感耦合等离子体质谱联用仪（HPLC-ICP-MS）测定和监测海鲜中的六种砷物种，包括亚砷酸盐（AsIII）、砷酸盐（AsV）、一甲基砷酸（MMA）、二甲基砷酸（DMA）、砷胆碱（AsC）和砷甜菜碱（AsB）。我们优化了用于砷物种分离和提取的色谱条件和样品制备方法，并使用有证标准物质和实际样品对该方法的准确性和重现性进行了验证。在12分钟内实现了砷物种的基线分离，并选择柱温箱热萃取进行样品制备。该方法对0.1至100 μg/L的砷物种显示出良好的线性，回收率在92%至123%之间，日内精密度为0.9 - 5.0%，日间精密度为1.1 - 19.4%。该方法进一步用于测定来自中国广东的109份软体动物、195份甲壳类动物和195份海鱼样品中的砷物种。在不同的海鲜类别中观察到了独特的砷物种分布模式。AsB始终是所有样品中的主要物种，检测频率高于97.9%，占总砷的90%以上，其他砷物种在其出现情况和浓度上表现出明显差异。
