Sahoo Swapnil, Rahaman Abdur
School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Odisha, India.
Homi Bhabha National Institute, Mumbai, India.
Methods Mol Biol. 2025;2958:71-81. doi: 10.1007/978-1-0716-4714-1_5.
The nuclear membrane undergoes continuous remodelling during the life cycle of all eukaryotic cells. The endoplasmic reticulum remains continuous with the nuclear membrane and regulates this nuclear remodelling process. Here in this chapter, we describe in detail a protocol for high-speed imaging of nuclear membrane/endoplasmic reticulum dynamic structure in live cell using fluorescent endoplasmic reticulum resident marker (GFP-KDEL). We used super-resolution microscope to capture this dynamic event. This protocol will help visualize the dynamic changes in nuclear membrane/endoplasmic reticulum structure at different cell cycle stages and understand the role of various cellular factors in this process.
在所有真核细胞的生命周期中，核膜经历持续重塑。内质网与核膜保持连续，并调节这种核重塑过程。在本章中，我们详细描述了一种使用荧光内质网驻留标记物（GFP-KDEL）对活细胞中核膜/内质网动态结构进行高速成像的方案。我们使用超分辨率显微镜来捕捉这一动态事件。该方案将有助于可视化核膜/内质网结构在不同细胞周期阶段的动态变化，并了解各种细胞因子在此过程中的作用。
