Fast Super-Resolution Live Cell Imaging of Nuclear Membrane-Endoplasmic Reticulum Dynamics.

The nuclear membrane undergoes continuous remodelling during the life cycle of all eukaryotic cells. The endoplasmic reticulum remains continuous with the nuclear membrane and regulates this nuclear remodelling process. Here in this chapter, we describe in detail a protocol for high-speed imaging of nuclear membrane/endoplasmic reticulum dynamic structure in live cell using fluorescent endoplasmic reticulum resident marker (GFP-KDEL). We used super-resolution microscope to capture this dynamic event. This protocol will help visualize the dynamic changes in nuclear membrane/endoplasmic reticulum structure at different cell cycle stages and understand the role of various cellular factors in this process.