Santos Ingredy Carolline de Jesus, Taconeli Cesar Augusto, Sabino Ester Cerdeira, Ribeiro Antonio Luiz Pinho, Souza Andréia Brito de, Sena Ana Beatriz Cardoso, Cruz Dardiane Santos, Leite Sâmara Fernandes, Santos Ana Clara de Jesus, Lacerda Amanda Mota, Fernandes Luciano de Freitas, Haikal Desirée Sant'Ana, Romero Israel Molina, Araújo Diego Dias de, Ferreira Ariela Mota
Universidade Estadual de Montes Claros, Programa de Pós-Graduação em Ciências da Saúde, Montes Claros, Minas Gerais, Brazil.
Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
Rev Inst Med Trop Sao Paulo. 2025 Aug 18;67:e55. doi: 10.1590/S1678-9946202567055. eCollection 2025.
This study aims to evaluate strategies for diagnosing Chagas disease (CD) using rapid tests and risk questions in an endemic area. This is an analytical cross-sectional study conducted with 751 individuals from two municipalities in an endemic region for CD in the North of Minas Gerais State, Brazil. Participants answered a questionnaire with personal information and the risk criteria for CD infection recommended by the Clinical Protocol and Therapeutic Guidelines for Chagas disease (PCDT). Subsequently, they underwent capillary blood collection for the rapid diagnostic test (RDT). Individuals with a positive RDT result, along with negative controls, underwent venipuncture for serological testing. The mean age of participants was 51.4 (±18.2) years, most were female (n=434/57.8%). In the RDT, 699 (93.1%) individuals tested negative; of these, 109 (15.6%) underwent serology and 4 (3.7%) tested positive. Among the 52 (6.9%) individuals with a positive RDT result, 48 (94.1%) had their result confirmed by the serological tests. RDT result, age, and risk factors-except for blood transfusion before 1992-were statistically associated with positive serology for CD. The RDT demonstrated high sensitivity (0.92; 95% CI: 0.81-0.92) and specificity (0.97; 95%CI: 0.92-0.99).
本研究旨在评估在流行地区使用快速检测和风险问题诊断恰加斯病（CD）的策略。这是一项分析性横断面研究，对巴西米纳斯吉拉斯州北部恰加斯病流行地区两个市的751名个体进行。参与者回答了一份包含个人信息以及恰加斯病临床方案和治疗指南（PCDT）推荐的CD感染风险标准的问卷。随后，他们接受毛细血管采血进行快速诊断检测（RDT）。RDT结果呈阳性的个体以及阴性对照者接受静脉穿刺进行血清学检测。参与者的平均年龄为51.4（±18.2）岁，大多数为女性（n = 434/57.8%）。在RDT检测中，699名（93.1%）个体检测为阴性；其中，109名（15.6%）接受了血清学检测，4名（3.7%）检测呈阳性。在RDT结果呈阳性的52名（6.9%）个体中，48名（94.1%）的结果经血清学检测得到证实。RDT结果、年龄以及除1992年前输血外的风险因素与CD血清学阳性在统计学上相关。RDT显示出高敏感性（0.92；95%CI：0.81 - 0.92）和特异性（0.97；95%CI：0.92 - 0.99）。
